二胺氧化酶活性及其测定的相关方面。

Aspects on diamine oxidase activity and its determination.

作者信息

Andersson A C, Henningsson S, Persson L, Rosengren E

出版信息

Acta Physiol Scand. 1978 Feb;102(2):159-66. doi: 10.1111/j.1748-1716.1978.tb06059.x.

DOI:10.1111/j.1748-1716.1978.tb06059.x

Abstract

Homogenate of guinea-pig liver and human placenta, tissues known to be rich in diamine oxidase, were incubated with 14C-putrescine and the metabolites formed were determined. In the incubate of guinea-pig liver, the major metabolites were GABA and some unidentified compound(s); delta1-pyrroline and 14CO2 were also obtained. In both the maternal and fetal parts of human placenta, radioactive GABA and the unidentified compound(s) as well as delta1-pyrroline were found. The results indicate that GABA is an important intermediate in putrescine metabolism. Determination of the amount of delta1-pyrroline is thus not well suited as a measure of the diamine oxidase activity in tissues.

摘要

已知富含二胺氧化酶的豚鼠肝脏和人胎盘组织匀浆与14C-腐胺一起孵育，并测定形成的代谢产物。在豚鼠肝脏孵育物中，主要代谢产物是γ-氨基丁酸（GABA）和一些未鉴定的化合物；还得到了δ1-吡咯啉和14CO2。在人胎盘的母体和胎儿部分均发现了放射性GABA、未鉴定的化合物以及δ1-吡咯啉。结果表明，GABA是腐胺代谢的重要中间产物。因此，测定δ1-吡咯啉的量不太适合作为组织中二胺氧化酶活性的指标。