猪肾二胺氧化酶催化对二甲基氨基甲基苄胺的氧化反应。一种分光光度法测定的新方法。
Oxidation of p-dimethylaminomethylbenzylamine by pig kidney diamine oxidase. A new method for spectrophotometric assay.
作者信息
Bardsley W G, Crabbe M J, Shindler J S, Ashford J S
出版信息
Biochem J. 1972 May;127(5):875-9. doi: 10.1042/bj1270875.
Abstract
- The oxidation of some para-substituted benzylamines by diamine oxidase produces the corresponding aldehydes. This was studied to develop a spectrophotometric method for following the enzyme reaction, as the aldehydes produced absorb strongly at 250nm where the substrates are almost optically transparent. 2. p-Dimethylaminomethylbenzylamine was the most useful substrate and full details of its preparation are given. The synthesis of its related oxidation product, p-dimethylaminomethylbenzaldehyde, is also described. 3. The effects of variations in pH, ionic strength, temperature and oxygenation on the reaction are described and the usefulness of the method is illustrated by several applications and assessed by comparison with the standard spectrophotometric assay.
摘要
- 二胺氧化酶催化一些对取代苄胺氧化生成相应的醛。对此进行了研究以开发一种用于跟踪酶反应的分光光度法，因为生成的醛在250nm处有强烈吸收，而底物在该波长下几乎是光学透明的。2. 对二甲氨基甲基苄胺是最有用的底物，并给出了其制备的详细信息。还描述了其相关氧化产物对二甲氨基甲基苯甲醛的合成。3. 描述了pH、离子强度、温度和氧合变化对反应的影响，并通过几个应用实例说明了该方法的实用性，并与标准分光光度法进行比较对其进行了评估。
相似文献
1
Oxidation of p-dimethylaminomethylbenzylamine by pig kidney diamine oxidase. A new method for spectrophotometric assay.猪肾二胺氧化酶催化对二甲基氨基甲基苄胺的氧化反应。一种分光光度法测定的新方法。
Biochem J. 1972 May;127(5):875-9. doi: 10.1042/bj1270875.
2
Inhibition of pig kidney diamine oxidase by substrate analogues.底物类似物对猪肾二胺氧化酶的抑制作用。
Biochem J. 1972 Jun;128(2):253-63. doi: 10.1042/bj1280253.
3
Kinetics of the diamine oxidase reaction.二胺氧化酶反应的动力学
Biochem J. 1973 Mar;131(3):459-69. doi: 10.1042/bj1310459.
4
Colorimetric estimation of diamine oxidase activity.二胺氧化酶活性的比色测定
Anal Biochem. 1975 Mar;64(1):177-85. doi: 10.1016/0003-2697(75)90419-4.
5
Superoxide anion radical generation during the oxidation of various amines by diamine oxidase.二胺氧化酶氧化各种胺类过程中超氧阴离子自由基的产生
Free Radic Res. 1996 Mar;24(3):167-75. doi: 10.3109/10715769609088014.
6
Synthesis and oxidation of aminoalkyl-onium compounds by pig kidney diamine oxidase.猪肾二胺氧化酶对氨基烷基鎓化合物的合成与氧化作用。
Biochem J. 1971 May;122(4):557-67. doi: 10.1042/bj1220557.
7
Oxidative deamination of 2-hydroxy derivatives of putrescine and cadaverine by pea-seedling and pig-kidney diamine oxidase.腐胺和尸胺的2-羟基衍生物经豌豆幼苗和猪肾二胺氧化酶进行氧化脱氨作用。
Biochim Biophys Acta. 1967 Mar 22;136(2):258-64. doi: 10.1016/0304-4165(67)90070-0.
8
The inhibition of human placental diamine oxidase by substrate analogues.底物类似物对人胎盘二胺氧化酶的抑制作用。
Biochem J. 1974 Apr;139(1):183-9. doi: 10.1042/bj1390183.
9
[Oxidation of p-nitrobenzylamine and p-dimethylaminomethylbenzylamine by amine oxidases from the human placenta and serum].[人胎盘和血清中的胺氧化酶对对硝基苄胺和对二甲氨基甲基苄胺的氧化作用]
Vopr Med Khim. 1986 Mar-Apr;32(2):118-25.
10
A reinvestigation of the substrate specificity of pig kidney diamine oxidase.猪肾二胺氧化酶底物特异性的重新研究。
Biochem J. 1970 Mar;117(1):169-76. doi: 10.1042/bj1170169.
引用本文的文献
1
Discovery of a sensitive, selective, and tightly binding fluorogenic substrate of bovine plasma amine oxidase.牛血浆胺氧化酶灵敏、特异且紧密结合的荧光底物的发现。
J Org Chem. 2009 Jan 2;74(1):339-50. doi: 10.1021/jo8018945.
2
Electrostatic control of oxidative deamination catalysed by bovine serum amine oxidase.牛血清胺氧化酶催化的氧化脱氨作用的静电控制
Biochem J. 1994 Apr 1;299 ( Pt 1)(Pt 1):317-20. doi: 10.1042/bj2990317.
3
Purification and characterization of pea seedling amine oxidase for crystallization studies.用于结晶研究的豌豆幼苗胺氧化酶的纯化与特性分析
Plant Physiol. 1994 Nov;106(3):1205-11. doi: 10.1104/pp.106.3.1205.
4
Summary of an international workshop on diamine oxidase held at the Department of Biogenic Amines, Polish Academy of Sciences, Lodz, Poland, June 7-8, 1979.1979年6月7日至8日在波兰罗兹市波兰科学院生物胺系举办的二胺氧化酶国际研讨会综述。
Agents Actions. 1981 Apr;11(1-2):3-8. doi: 10.1007/BF01991445.
5
Inhibition of pig kidney diamine oxidase by substrate analogues.底物类似物对猪肾二胺氧化酶的抑制作用。
Biochem J. 1972 Jun;128(2):253-63. doi: 10.1042/bj1280253.
6
The inhibition of human placental diamine oxidase by substrate analogues.底物类似物对人胎盘二胺氧化酶的抑制作用。
Biochem J. 1974 Apr;139(1):183-9. doi: 10.1042/bj1390183.
7
The amine oxidases of human placenta and pregnancy plasma.人胎盘和妊娠血浆中的胺氧化酶。
Biochem J. 1974 Apr;139(1):169-81. doi: 10.1042/bj1390169.
8
Inhibition of enzymes by metal ion-chelating reagents. The action of copper-chelating reagents on diamine oxidase.金属离子螯合试剂对酶的抑制作用。铜螯合试剂对二胺氧化酶的作用。
Biochem J. 1974 Jan;137(1):61-6. doi: 10.1042/bj1370061.
9
Kinetics of the diamine oxidase reaction.二胺氧化酶反应的动力学
Biochem J. 1973 Mar;131(3):459-69. doi: 10.1042/bj1310459.
10
Transient kinetics of copper-containing lentil (Lens culinaris) seedling amine oxidase.含铜兵豆（Lens culinaris）幼苗胺氧化酶的瞬态动力学
Biochem J. 1985 Dec 15;232(3):923-6. doi: 10.1042/bj2320923.
本文引用的文献
1
Determination of diamine oxidase activity by liquid scintillation counting.通过液体闪烁计数法测定二胺氧化酶活性。
Arch Biochem Biophys. 1961 Nov;95:242-50. doi: 10.1016/0003-9861(61)90141-2.
2
Further studies of a spectrophotometric method for the determination of diamine oxidase activity.分光光度法测定二胺氧化酶活性的进一步研究
Biochim Biophys Acta. 1961 Mar 18;48:182-6. doi: 10.1016/0006-3002(61)90530-3.
3
Purification of amine oxidase from beef plasma.从牛血浆中纯化胺氧化酶。
J Biol Chem. 1954 Jun;208(2):645-61.
4
Synthesis and oxidation of aminoalkyl-onium compounds by pig kidney diamine oxidase.猪肾二胺氧化酶对氨基烷基鎓化合物的合成与氧化作用。
Biochem J. 1971 May;122(4):557-67. doi: 10.1042/bj1220557.
5
A reinvestigation of the substrate specificity of pig kidney diamine oxidase.猪肾二胺氧化酶底物特异性的重新研究。
Biochem J. 1970 Mar;117(1):169-76. doi: 10.1042/bj1170169.
Zotero 插件