Delmas C, Prère M F, Fayet O, Lareng M B, Dabernat H
Ann Inst Pasteur Microbiol (1985). 1985 Jul-Aug;136B(1):29-38. doi: 10.1016/s0769-2609(85)80003-x.
The enzymatic profile of "Neisseria polysacchareae" was determined by using chromogenic substrate (API System), and was compared to that of N. meningitidis, N. gonorrhoeae and N. lactamica. The tested classes of enzymes were aminopeptidase, proteases, esterases, lipases, glycosidases, phosphatase and phosphoamidase. "N. polysacchareae" exhibited various aminopeptidase and protease activities and a strong esterase activity. No lipase and glycosidase activities were detected by the tested substrates. The strains of "N. polysacchareae" differed from that of N. meningitidis in the presence of hydroxyproline aminopeptidase and the lack of gamma-glutamyl-transferase activity. Five strains harboured extrachromosomal elements. The plasmids were of 4.2 Kb in size in four cases and of more than 40 Kb in four cases. Three strains simultaneously harboured these two plasmids. This plasmid content is another characteristic of strains of this new taxon. No phenotypic modification was observed in plasmid-containing strains.
采用显色底物（API系统）测定了“多糖奈瑟菌”的酶谱，并将其与脑膜炎奈瑟菌、淋病奈瑟菌和乳糖奈瑟菌的酶谱进行了比较。所检测的酶类包括氨肽酶、蛋白酶、酯酶、脂肪酶、糖苷酶、磷酸酶和磷酸酰胺酶。“多糖奈瑟菌”表现出多种氨肽酶和蛋白酶活性以及较强的酯酶活性。在所检测的底物中未检测到脂肪酶和糖苷酶活性。“多糖奈瑟菌”菌株与脑膜炎奈瑟菌菌株的区别在于前者存在羟脯氨酸氨肽酶且缺乏γ-谷氨酰转移酶活性。五株菌株含有染色体外元件。四例中的质粒大小为4.2 kb，四例中的质粒大小超过40 kb。三株菌株同时含有这两种质粒。这种质粒含量是这个新分类单元菌株的另一个特征。在含质粒的菌株中未观察到表型改变。
