Lognonne J L, Ducousso R, Rocquet G, Kergonou J F
Biochimie. 1985 Sep;67(9):1015-21. doi: 10.1016/s0300-9084(85)80296-0.
The effects of whole-body gamma irradiation (8.4 Gy) were studied on arachidonic acid (AA) metabolism in rats' blood platelets, from day D + 1 to day D + 10 after irradiation. AA conversion into thromboxane B2 (TxB2) increased at D + 1 and then gradually decreased to very low values from D + 7 to D + 10. This decrease in the conversion of exogenous AA into TxB2 was due to a lower AA incorporation into platelets and not to a decrease of cyclooxygenase and thromboxane-synthetase activities. AA incorporation into membrane phospholipids of blood platelets was much more decreased than AA incorporation into whole platelets; moreover, the lipid composition of the platelet membranes was markedly modified after irradiation, which must have resulted in structural and functional changes in these membranes; from these effects of whole-body gamma irradiation on platelets, the latter's membranes appeared as a major site of in vivo radiation damage in these cells.
研究了全身γ射线照射（8.4 Gy）对大鼠血小板中花生四烯酸（AA）代谢的影响，观察时间为照射后第1天（D + 1）至第10天（D + 10）。照射后第1天，AA转化为血栓素B2（TxB2）增加，然后从第7天到第10天逐渐降至非常低的水平。外源性AA转化为TxB2的减少是由于AA掺入血小板减少，而不是由于环氧化酶和血栓素合成酶活性降低。与AA掺入整个血小板相比，AA掺入血小板膜磷脂的减少更为明显；此外，照射后血小板膜的脂质组成发生了明显改变，这必然导致了这些膜的结构和功能变化；从全身γ射线照射对血小板的这些影响来看，血小板膜似乎是这些细胞体内辐射损伤的主要部位。
