Molin G
Can J Microbiol. 1985 Sep;31(9):763-6. doi: 10.1139/m85-143.
The growth of Pseudomonas putida ATCC 11172 on L-asparagine, citrate, D-glucose, and L-lactate was followed in air and in 40% CO2 + air, using batch and carbon-limited continuous cultures. Batch cultures in air utilized a mixture of the carbon sources simultaneously. However, a change to 40% CO2 favoured the utilization of glucose. The maximum specific growth rate (mumax) in air was about 0.3 h-1 on glucose and 0.6 h-1 on the other carbon sources. In CO2, the mumax for glucose was reduced by 16% compared with almost 60-70% for the others. An order of preference for the different carbon sources in continuous cultures was determined by comparing the dilution rates at which the different carbon sources started to appear in the effluent. Glucose was the first compound to appear as the dilution rate increased (lowest preference when grown in air). In 40% CO2, the mumax for glucose was slightly higher than the others and the recorded preference for glucose in continuous culture was equal to that for citrate but was somewhat lower than that of lactate and asparagine. D-Gluconate and glucono-delta-lactone were produced as a step in the utilization of glucose. The D-gluconate production was enhanced by CO2.
在空气和40%二氧化碳+空气环境下，采用分批培养和碳源限制连续培养的方式，跟踪恶臭假单胞菌ATCC 11172在L-天冬酰胺、柠檬酸盐、D-葡萄糖和L-乳酸上的生长情况。在空气中进行的分批培养同时利用了多种碳源的混合物。然而，改为40%二氧化碳环境有利于葡萄糖的利用。在空气中，葡萄糖的最大比生长速率（μm ax）约为0.3 h-1，其他碳源的最大比生长速率为0.6 h-1。在二氧化碳环境中，葡萄糖的μm ax相比其他碳源降低了16%，而其他碳源的降低幅度接近60-70%。通过比较不同碳源开始出现在流出物中的稀释率，确定了连续培养中不同碳源的优先顺序。随着稀释率的增加，葡萄糖是第一个出现的化合物（在空气中生长时偏好最低）。在40%二氧化碳环境中，葡萄糖的μm ax略高于其他碳源，并且在连续培养中记录到的对葡萄糖的偏好与对柠檬酸盐的偏好相当，但略低于对乳酸盐和天冬酰胺的偏好。D-葡萄糖酸盐和葡萄糖酸-δ-内酯是葡萄糖利用过程中的产物。二氧化碳可提高D-葡萄糖酸盐的产量。
