Tomonaga S, Kobayashi K, Hagiwara K, Sasaki K, Sezaki K
Dev Comp Immunol. 1985 Fall;9(4):617-26. doi: 10.1016/0145-305x(85)90027-8.
Immunoglobulin (Ig), lymphoid tissues and Ig-forming cells of the Japanese bullhead shark, Heterodontus japonicus were analyzed biochemically, histologically and immunocytochemically. The serum of Heterodontus contains two Igs with different molecular weights one with 900 K and the other with 180 K daltons. Heavy chains of the two Igs showed an identical molecular weight of 68 K and the same antigenicity, indicating that the two Igs belong to the same class with different molecular structure. Light chains of Heterodontus Igs showed two distinct bands using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, one with the molecular weight of 25 K and the other with 22 K daltons. The latter finding indicates the possible existence of two light chain types in the Heterodontus Igs. White pulp of the spleen appeared as a well-developed lymphoid tissue accompanied large number of Ig-forming cells especially around blood vessels. Massive lymphocytic aggregations were found in the central area of the intestinal valves and certain lymphoid cells were demonstrated to be Ig-forming cells. Ig-forming cells were also observed in the epigonal organ, although the frequency was much less than in the former two tissues. Although the spleen is the major Ig-forming organ in Heterodontus japonicus, the valvular intestine and the epigonal organ also appear to share the function of Ig production.
对日本虎头鲨（Heterodontus japonicus）的免疫球蛋白（Ig）、淋巴组织和产生Ig的细胞进行了生化、组织学和免疫细胞化学分析。日本虎头鲨的血清中含有两种分子量不同的Ig，一种为900K，另一种为180K道尔顿。这两种Ig的重链显示出相同的分子量68K和相同的抗原性，表明这两种Ig属于同一类但分子结构不同。日本虎头鲨Ig的轻链在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中显示出两条不同的条带，一条分子量为25K，另一条为22K道尔顿。后一发现表明日本虎头鲨Ig中可能存在两种轻链类型。脾脏的白髓表现为发育良好的淋巴组织，伴有大量产生Ig的细胞，尤其是在血管周围。在肠瓣膜的中央区域发现大量淋巴细胞聚集，并且某些淋巴细胞被证明是产生Ig的细胞。在性腺旁器官中也观察到了产生Ig的细胞，尽管其频率远低于前两个组织。虽然脾脏是日本虎头鲨中主要的产生Ig的器官，但瓣膜肠和性腺旁器官似乎也具有产生Ig的功能。
