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用于检测I-III型干扰素及其抗体中和作用的高度敏感的报告细胞系。

Highly sensitive reporter cell line for detection of interferon types I-III and their neutralization by antibodies.

作者信息

Groen Kevin, Kuratli Roger, Sar Lauren, Vasou Andri, Huber Michael, Hughes David J, Hale Benjamin G

机构信息

Institute of Medical Virology, University of Zurich, Zurich, Switzerland.

Biomedical Sciences Research Complex, University of St. Andrews, St. Andrews, United Kingdom.

出版信息

Eur J Immunol. 2024 Dec;54(12):e2451325. doi: 10.1002/eji.202451325. Epub 2024 Oct 4.

DOI:10.1002/eji.202451325
PMID:39367550
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11628890/
Abstract

Interferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase-based reporter cell line capable of detecting the activities of IFN-Is, IFN-II, and IFN-IIIs. The reporter cell line exhibits a 125- to 2000-fold higher sensitivity to IFNs than a commonly used alternative biological reporter system and allows for a rapid and simple live-cell workflow for detecting low titer amounts of neutralizing anti-IFN antibodies.

摘要

干扰素（IFNs）是先天免疫防御的关键组成部分，可限制病毒疾病的严重程度。为了推进对干扰素及其被致病性自身抗体中和的研究，我们构建了一种基于海肾荧光素酶的报告细胞系，该细胞系能够检测I型干扰素、II型干扰素和III型干扰素的活性。与常用的替代生物报告系统相比，该报告细胞系对干扰素的敏感性高125至2000倍，并允许采用快速简单的活细胞工作流程来检测低滴度的中和抗干扰素抗体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf3/11628890/5cbc4a815a21/EJI-54-2451325-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf3/11628890/ea1420f61492/EJI-54-2451325-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf3/11628890/5cbc4a815a21/EJI-54-2451325-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf3/11628890/ea1420f61492/EJI-54-2451325-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddf3/11628890/5cbc4a815a21/EJI-54-2451325-g001.jpg

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