卡介苗甲醇提取残渣（MER）的特性分析

Characterization of methanol extraction residue (MER) from Bacillus Calmette-Guérin (BCG).

作者信息

Shaikh B, Zielinski W L, Douros J D

出版信息

J Antibiot (Tokyo). 1979 Oct;32(10):1011-5. doi: 10.7164/antibiotics.32.1011.

DOI:10.7164/antibiotics.32.1011

Abstract

Analysis of methanol extraction residue (MER) from Bacillus Calmette-Guerin (BCG) was carried out to determine some specific chemical compositional characteristics. Samples of MER were found to contain approximately 40% protein and/or peptide, 3% soluble lipids, 17% bound lipids, 8% elemental nitrogen, and less than 2% mycolic acids. Amino acid analysis showed the presence of alanine, glycine and glutamic acid as the major amino acids. The data are reported in terms of the range found for each constituent over the samples analyzed. Somewhat consistent results were obtained between different MER preparations, but notable compositional variations were observed in samples of MER suspensions.

摘要

对卡介苗（BCG）的甲醇提取残渣（MER）进行了分析，以确定其一些特定的化学组成特征。发现MER样品含有约40%的蛋白质和/或肽、3%的可溶性脂质、17%的结合脂质、8%的元素氮以及少于2%的分枝菌酸。氨基酸分析表明，丙氨酸、甘氨酸和谷氨酸是主要氨基酸。数据报告的是在所分析样品中每种成分的发现范围。不同的MER制剂之间获得了 somewhat consistent 的结果，但在MER悬浮液样品中观察到了显著的成分变化。