Coll Food Sci & Light Ind, Nanjing Tech University, Nanjing, 211800, China.
Mikrochim Acta. 2024 Oct 5;191(11):648. doi: 10.1007/s00604-024-06685-7.
Real-time, label-free detection of gram-negative bacteria with high selectivity and sensitivity is demonstrated using a bipolar electrode-electrochemiluminescence (BPE-ECL) platform. This platform utilizes anode luminescence and cathode modification of antimicrobial peptides (AMPs) to effectively capture bacteria. Magainin I, basic AMP from Xenopus skin, boasting an α-helix structure, exhibits a preferential affinity for the surface of gram-negative pathogens. The covalent attachment of the peptide's C-terminal carboxylic acid to the free amines of a previously thiolated linker ensures its secure immobilization onto the surface of the interdigitated gold-plated cathode of BPE. The AMP-modified BPE sensor, when exposed to varying concentrations of gram-negative bacteria, produces reproducible ECL intensities, allowing for the detection of peptide-bacteria interactions within the range 1 to 10 CFU mL. Furthermore, this AMP-modified BPE sensor demonstrates a selective capacity to detect Escherichia coli O157:H7 amidst other gram-negative strains, even at a concentration of 1-CFU mL. This study underscores the high selectivity of Magainin I in bacterial detection, and the AMP-modified BPE-ECL system holds significant promise for rapid detection of gram-negative bacteria in various applications. The AMP-modified BPE sensor generated reproducible ECL intensity that detected peptide-bacteria interactions in the range 1 to 10 CFU mL. The AMP-modified BPE sensor also selectively detected E. coli O157:H7 from other gram-negative strains at a concentration of 1-CFU mL. In this paper, AMP demonstrated high selectivity in bacterial detection. The AMP-modified BPE-ECL system prepared has a great potential for application in the field of rapid detection of gram-negative bacteria.
使用双极电极-电化学发光（BPE-ECL）平台，展示了对革兰氏阴性菌具有高选择性和灵敏度的实时、无标记检测。该平台利用阳极发光和抗菌肽（AMPs）的阴极修饰来有效捕获细菌。Magainin I，一种来自非洲爪蟾皮肤的基本 AMP，具有α-螺旋结构，对革兰氏阴性病原体的表面表现出优先亲和力。肽的 C 末端羧酸与先前硫醇化接头的游离胺共价连接，确保其牢固固定在 BPE 的叉指镀金阴极的表面上。当 AMP 修饰的 BPE 传感器暴露于不同浓度的革兰氏阴性菌时，会产生可重复的 ECL 强度，允许在 1 至 10 CFU mL 的范围内检测肽-细菌相互作用。此外，这种 AMP 修饰的 BPE 传感器在检测其他革兰氏阴性菌株中的大肠杆菌 O157:H7 时表现出选择性，即使在 1-CFU mL 的浓度下也是如此。这项研究强调了 Magainin I 在细菌检测中的高选择性，并且 AMP 修饰的 BPE-ECL 系统在各种应用中快速检测革兰氏阴性细菌具有重要意义。AMP 修饰的 BPE 传感器产生了可重复的 ECL 强度，可检测 1 至 10 CFU mL 范围内的肽-细菌相互作用。AMP 修饰的 BPE 传感器还可选择性地从其他革兰氏阴性菌株中检测到浓度为 1-CFU mL 的大肠杆菌 O157:H7。在本文中，AMP 表现出在细菌检测中的高选择性。制备的 AMP 修饰的 BPE-ECL 系统在革兰氏阴性菌快速检测领域具有很大的应用潜力。
