用于从痰液样本中检测结核分枝杆菌的改良快速放射测量法。
Modified rapid radiometric method for detection of Mycobacterium tuberculosis from sputum samples.
作者信息
Shah D H, Devdhar M N, Ganatra R D, Kale P N, Virdi S S, Deshmukh M D
出版信息
Int J Nucl Med Biol. 1985;12(4):333-5. doi: 10.1016/0047-0740(85)90190-1.
A modified, simple and radiometric method for early detection of M. tuberculosis from sputum samples has been developed using a biphasic vial system for detection of 14C-CO2 produced by the metabolism of 14C-U-acetate on glycerol-free Lowenstein-Jensen medium (LJM). Of the 84 smear positive sputum samples examined, 85.7% and 86.9% were scored positive by radiometric and visual methods respectively. The detection rates at 1st, 2nd and 3rd week of the test were 53.3%, 60.7% and 82.1% by radiometry and 1.2%, 11.9% and 54.8% by visual methods respectively. The mean detection time was 10.7 days by the radiometric and 21.0 days by the visual method. An average replication time of primary culture from 54 sputum samples was 25.58 +/- 6.92 h (range 10.0-39.1 h).
已开发出一种改良的、简单的放射性检测方法，用于从痰标本中早期检测结核分枝杆菌。该方法使用双相瓶系统，在无甘油的罗氏培养基（LJM）上检测由14C-U-乙酸盐代谢产生的14C-CO2。在检测的84份涂片阳性痰标本中，放射性检测法和肉眼观察法的阳性率分别为85.7%和86.9%。检测第1、2和3周时，放射性检测法的检出率分别为53.3%、60.7%和82.1%，肉眼观察法的检出率分别为1.2%、11.9%和54.8%。放射性检测法的平均检测时间为10.7天，肉眼观察法为21.0天。对54份痰标本进行原代培养，其平均繁殖时间为25.58 +/- 6.92小时（范围为10.0 - 39.1小时）。
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