Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
Microb Cell Fact. 2024 Oct 5;23(1):266. doi: 10.1186/s12934-024-02530-x.
Hazardous synthetic dye wastes have become a growing threat to the environment and public health. Fungal enzymes are eco-friendly, compatible and cost-effective approach for diversity of applications. Therefore, this study aimed to screen, optimize fermentation conditions, and characterize laccase from fungal endophyte with elucidating its ability to decolorize several wastewater dyes.
A new fungal endophyte capable of laccase-producing was firstly isolated from cladodes of Opuntia ficus-indica and identified as T. harzianum AUMC14897 using ITS-rRNA sequencing analysis. Furthermore, the response surface methodology (RSM) was utilized to optimize several fermentation parameters that increase laccase production. The isolated laccase was purified to 13.79-fold. GFC, SDS-PAGE revealed laccase molecular weight at 72 kDa and zymogram analysis elucidated a single band without any isozymes. The peak activity of the pure laccase was detected at 50 °C, pH 4.5, with thermal stability up to 50 °C and half life span for 4 h even after 24 h retained 30% of its activity. The K and V values were 0.1 mM, 22.22 µmol/min and activation energy (E) equal to 5.71 kcal/mol. Furthermore, the purified laccase effectively decolorized various synthetic and real wastewater dyes.
Subsequently, the new endophytic strain produces high laccase activity that possesses a unique characteristic, it could be an appealing candidate for both environmental and industrial applications.
危险合成染料废物对环境和公共健康构成了日益严重的威胁。真菌酶是一种环保、兼容且具有成本效益的方法，可应用于多种领域。因此，本研究旨在筛选、优化发酵条件，并对具有阐明其对多种废水染料脱色能力的真菌内生菌来源的漆酶进行特性分析。
首次从仙人掌的节段中分离到一种能够产漆酶的新型真菌内生菌，并通过 ITS-rRNA 测序分析鉴定为哈茨木霉 AUMC14897。此外，利用响应面法（RSM）优化了几个可提高漆酶产量的发酵参数。分离出的漆酶经纯化后达到 13.79 倍。GFC、SDS-PAGE 显示漆酶的分子量为 72 kDa，酶谱分析表明没有同工酶，只有一条单一的带。纯漆酶的最适活性在 50°C、pH4.5 下检测到，热稳定性高达 50°C，半衰期为 4 小时，24 小时后仍保留 30%的活性。K 和 V 值分别为 0.1 mM、22.22 µmol/min 和活化能（E）等于 5.71 kcal/mol。此外，纯化的漆酶能有效脱色各种合成和实际废水染料。
随后，新的内生菌株产生了高漆酶活性，具有独特的特性，它可能是环境和工业应用的有吸引力的候选者。
