Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada; Sinclair Cancer Research Institute, Queen's University, Kingston, Ontario, Canada.
F S Sci. 2024 Nov;5(4):335-341. doi: 10.1016/j.xfss.2024.10.001. Epub 2024 Oct 5.
To determine whether tertiary lymphoid structures (TLSs), which reflect organized immune cell aggregates present in non-lymphoid tissues, are consistent features of endometriosis lesions.
Detailed histopathological analysis of endometrial and lesion tissue from patients with endometriosis and controls was performed. Multiplex immunofluorescence on select samples was then conducted to identify canonical cell populations present within TLSs: CD3 and CD8 T-cells, CD79a B-cells, CD208 dendritic cells, CD21 follicular dendritic cells, and PNAd high endothelial venules.
PATIENT(S): Patients with histologically confirmed endometriosis (N = 113; 44.3 ± 6.0) and control individuals (N = 110; 44.6 ± 7.1).
Not applicable.
MAIN OUTCOME MEASURE(S): Detection of TLSs as characterized by the presence of all canonical cell types that constitute TLS and structure morphology.
RESULT(S): Of the selected samples (N = 18; 6 ectopic/eutopic/control), mature TLSs were identified in 3 ectopic tissue samples present on the ovary and fallopian tube, with immature TLSs (lacking follicular dendritic cell networks and high endothelial venules) present throughout eutopic and control endometrial samples.
These findings demonstrate the presence of TLSs across various endometriosis phenotypes, prompting further research into their significance within disease pathophysiology and the prognostic implications for patients.
确定三型淋巴结构（TLSs）是否为子宫内膜异位症病变的固有特征，TLSs 反映了非淋巴组织中存在的有组织的免疫细胞聚集。
对子宫内膜异位症患者和对照者的子宫内膜和病变组织进行详细的组织病理学分析。然后对选择的样本进行多重免疫荧光分析，以鉴定 TLS 内存在的典型细胞群：CD3 和 CD8 T 细胞、CD79a B 细胞、CD208 树突状细胞、CD21 滤泡树突状细胞和 PNAd 高内皮静脉。
经组织学证实的子宫内膜异位症患者（N=113；44.3±6.0）和对照者（N=110；44.6±7.1）。
不适用。
通过存在构成 TLS 的所有典型细胞类型和结构形态来检测 TLSs。
在选择的样本（N=18；6 个异位/同源/对照）中，在卵巢和输卵管上的 3 个异位组织样本中鉴定出成熟的 TLSs，在同源和对照的子宫内膜样本中存在不成熟的 TLSs（缺乏滤泡树突状细胞网络和高内皮静脉）。
这些发现表明 TLSs 存在于各种子宫内膜异位症表型中，促使进一步研究它们在疾病病理生理学中的意义及其对患者的预后影响。
