Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, China.
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
New Phytol. 2024 Dec;244(6):2396-2412. doi: 10.1111/nph.20174. Epub 2024 Oct 6.
Phosphate (Pi) availability is well known to regulate plant root growth. However, it remains largely unknown how flavonoid synthesis participates in affecting plant root growth in response to Pi starvation. In the study, the crystal structure of a plant protein phosphatase, GmHAD1-2, was dissected using X-ray crystallography for the first time. It was revealed that GmHAD1-2 contained a modified Rossmannoid class of α/β folds with three layered α/β sandwich. Transcripts of GmHAD1-2 were increased by Pi starvation in soybean roots, especially in lateral root tips. GmHAD1-2 suppression or overexpression significantly influenced soybean lateral root length and number, as well as phosphorus (P) content. Furthermore, GmHAD1-2 was found to interact with a chalcone reductase, GmCHR1. Suppression of GmHAD1-2 significantly changed the flavonoid biosynthesis pathway in soybean roots. Taken together, the results highlight that GmHAD1-2 can regulate soybean root growth by influencing flavonoid metabolism.
磷酸盐（Pi）的可用性是众所周知的，它可以调节植物根系的生长。然而，黄酮类化合物的合成如何参与影响植物根系对磷饥饿的反应，在很大程度上仍然未知。在这项研究中，首次使用 X 射线晶体学解析了一种植物蛋白磷酸酶 GmHAD1-2 的晶体结构。结果表明，GmHAD1-2 包含一个改良的 Rossmannoid 类的α/β折叠，具有三层α/β三明治结构。大豆根系在磷饥饿时，GmHAD1-2 的转录本增加，特别是在侧根根尖。GmHAD1-2 的抑制或过表达显著影响大豆侧根的长度和数量，以及磷（P）含量。此外，还发现 GmHAD1-2 与查尔酮还原酶 GmCHR1 相互作用。GmHAD1-2 的抑制显著改变了大豆根系中的类黄酮生物合成途径。总之，这些结果强调了 GmHAD1-2 可以通过影响类黄酮代谢来调节大豆根系的生长。
