Ibba A, Rosa A, Maxia A, Fais S, Orrù G, Porcedda S, Piras A
Department of Chemical and Geological Sciences, University of Cagliari, Monserrato, CA, Italy.
Department of Surgical Sciences, University of Cagliari, Cagliari, Italy.
Nat Prod Res. 2024 Oct 7:1-6. doi: 10.1080/14786419.2024.2409983.
In this study, we continue our research on Schott extracts obtained by supercritical fluid extraction (SFE) using CO as solvent. Extraction experiments were carried out at pressures of 300 bar and a temperature of 40 °C, SFE [300:40], on mature fruits of . The fatty acid (FA) profile of extracts was assessed by reversed-phase HPLC-DAD/ELSD analysis. The most represented fatty acids in the extract were linoleic acid (386 mg/g), oleic acid (210 mg/g), linolenic acid (112 mg/g), palmitic acid (48 mg/g), and stearic acid (40 mg/g). SFE extraction showed a total FA amount of 795.8 mg/g of dry weight. The chemical and biological characteristics of this extract were compared with those obtained with different extraction methods. Following the diffusion-susceptibility test, three fruit extracts, SFE [300:40], Sx, and MEtOH, didn't show an inhibition profile against . Kirby-Bauer analysis showed only the methanolic extract demonstrated antimicrobial activity with 11-mm inhibition diameter.
在本研究中，我们继续对以CO作为溶剂通过超临界流体萃取（SFE）获得的肖特提取物展开研究。萃取实验在300巴的压力和40°C的温度下进行，即SFE [300:40]，以……的成熟果实为原料。提取物的脂肪酸（FA）谱通过反相HPLC-DAD/ELSD分析进行评估。提取物中含量最多的脂肪酸为亚油酸（386毫克/克）、油酸（210毫克/克）、亚麻酸（112毫克/克）、棕榈酸（48毫克/克）和硬脂酸（40毫克/克）。SFE萃取显示干重下总FA含量为795.8毫克/克。将该提取物的化学和生物学特性与用不同萃取方法获得的提取物进行了比较。经过扩散敏感性测试，三种果实提取物，即SFE [300:40]、Sx和甲醇提取物，未显示出对……的抑制谱。 Kirby-Bauer分析表明只有甲醇提取物表现出抗菌活性，抑菌直径为11毫米。
