Xie Lele, Ma Yushou, Wang Yanlong, Ma Yuan, Wang Xiaoli
Qinghai Academy of Animal and Veterinary Science Qinghai University Xining Qinghai China.
Academy of Animal and Veterinary Science Qinghai University Xining Qinghai China.
Ecol Evol. 2024 Oct 3;14(10):e70361. doi: 10.1002/ece3.70361. eCollection 2024 Oct.
About 35% of grassland in Sanjiangyuan area of China has degenerated into black-soil mountain. Artificial grassland is considered to be an effective measure to alleviate the severely degraded grassland in the alpine region of the three rivers and has been widely used. However, the pattern, potential function, and changes of carbon and nitrogen contents of soil microorganisms in degraded grassland in Heimushan by planting artificial grassland are still unclear. In this study, mixed-sown artificial alpine grassland (AG) was the focus of our study, whereas degraded black-soil mountain grassland (BG) and natural alpine grassland (NG) served as controls. Illumina 16S and ITS gene sequence analyses were used to analyze the community structure of the soil bacteria and fungi. The functional groups of NG, AG, and BG were predicted using the FAPROTAX and FUNGuild databases. In addition, the levels of soil carbon, nitrogen, and soil enzyme activities were evaluated. The results indicated a significant increase in the aboveground biomass of BG due to the planting artificial grassland. Moreover, the contents of total carbon (TC), total nitrogen (TN), ammonium nitrogen ( ), microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), and leucine aminopeptidase (LAP) increased in the soil. Planting artificial grasslands changed the composition of bacterial and fungal communities. Among these, the bacterial community was more sensitive to planting artificial grasslands. The relative abundance of bacterial functional groups involved in carbon and nitrogen cycling changed significantly, suggesting that bacteria may play a role in regulating nutrient cycling during artificial grassland planting. Soil TC, TN, LAP, and affected the microbial community structure related to carbon and nitrogen. and β-1,4-glucosidase were carbon and nitrogen factors, respectively, that affected functional changes in fungi. These results indicate that planting artificial grasslands can effectively enhance the productivity of degraded black-soil mountain and regulate soil microbial communities and soil physical and chemical properties.
中国三江源地区约35%的草原已退化为黑土滩。人工草地被认为是缓解三江源高寒地区严重退化草地的有效措施，并已得到广泛应用。然而，通过种植人工草地，黑土山退化草地土壤微生物的碳氮含量格局、潜在功能及变化仍不清楚。本研究以混播人工高寒草地（AG）为研究重点，以退化黑土滩草地（BG）和天然高寒草地（NG）作为对照。采用Illumina 16S和ITS基因序列分析方法分析土壤细菌和真菌的群落结构。利用FAPROTAX和FUNGuild数据库预测NG、AG和BG的功能群。此外，还评估了土壤碳、氮水平及土壤酶活性。结果表明，种植人工草地使BG的地上生物量显著增加。此外，土壤中总碳（TC）、总氮（TN）、铵态氮（ ）、微生物生物量碳（MBC）、微生物生物量氮（MBN）和亮氨酸氨肽酶（LAP）含量增加。种植人工草地改变了细菌和真菌群落的组成。其中，细菌群落对种植人工草地更为敏感。参与碳氮循环的细菌功能群相对丰度发生显著变化，表明细菌可能在人工草地种植过程中调节养分循环发挥作用。土壤TC、TN、LAP和 影响与碳氮相关的微生物群落结构。 和β-1,4-葡萄糖苷酶分别是影响真菌功能变化的碳和氮因素。这些结果表明，种植人工草地可以有效提高退化黑土滩的生产力，并调节土壤微生物群落及土壤理化性质。
