Casenave Clémence, Mangeon Pastori Carine, Cramail Henri, Grelier Stéphane
University of Bordeaux, CNRS, Bordeaux INP, LCPO, UMR 5629, 16 Avenue Pey-Berland, F-33600 Pessac, France.
Evertree, Les rives de l'Oise, 60201 Compiègne, France.
ACS Omega. 2024 Sep 20;9(39):40676-40686. doi: 10.1021/acsomega.4c04944. eCollection 2024 Oct 1.
Alternative biobased adhesive formulations are currently being investigated to replace urea-formaldehyde (UF) as wood panel adhesives. In this regard, oilseed meals are valuable alternatives, as it is anticipated that the sticky potential of these meals is linked to their protein content. This work focuses on the protein parameters (primary and/or secondary structures) that could impact the adhesiveness of sunflower meals. The proteins contained in these meals were first separated from the other components and identified using electrophoresis. Oilseed meals contain several families of proteins: globulins, albumins, prolamins, and oleosins. Sunflower meal is mainly composed of globulin (53%) and albumin (45%). The protein structures have then been either oxidized with HO (in the presence or not of NaOH) or physically treated by microwave (MW). The oxidation treatment cleaves the protein backbone and creates smaller peptides, while the MW process converts α-helices into random coils. The adhesive potential of these treated proteins was evaluated by using shear tests onto wood panels. The results demonstrate that the primary and secondary structures of globulins are key parameters toward the sunflower protein meal adhesivity.
目前正在研究替代生物基粘合剂配方，以取代脲醛（UF）作为人造板粘合剂。在这方面，油籽粕是有价值的替代品，因为预计这些粕的粘性潜力与其蛋白质含量有关。这项工作聚焦于可能影响向日葵粕粘附性的蛋白质参数（一级和/或二级结构）。首先将这些粕中含有的蛋白质与其他成分分离，并通过电泳进行鉴定。油籽粕含有几个蛋白质家族：球蛋白、白蛋白、醇溶蛋白和油体蛋白。向日葵粕主要由球蛋白（53%）和白蛋白（45%）组成。然后，蛋白质结构要么用HO（在有或没有NaOH的情况下）氧化，要么用微波（MW）进行物理处理。氧化处理会切断蛋白质主链并产生较小的肽段，而微波处理会将α-螺旋转化为无规卷曲。通过在人造板上进行剪切试验来评估这些处理过的蛋白质的粘附潜力。结果表明，球蛋白的一级和二级结构是影响向日葵蛋白粕粘附性的关键参数。
