兔抗人β2微球蛋白抗体：一种T细胞有丝分裂原。
Rabbit anti-human beta 2 microglobulin antibodies: a T-cell mitogen.
作者信息
Hammarström L, Smith C I
出版信息
Scand J Immunol. 1985 Dec;22(6):677-81. doi: 10.1111/j.1365-3083.1985.tb01930.x.
Rabbit anti-beta 2 microglobulin antibodies have been described as a potent mitogen for human B lymphocytes. However, when fractionated after activation, only the T-cell enriched population is actively dividing. The induction of proliferation in purified T cells requires the presence of non-T cells. Daudi cells (which do not express beta 2 microglobulin on their cell surface) were shown to produce 'macrophage replacing factors' which supported anti-beta 2-induced T cell division. Non-T cells could be effectively replaced by addition of interleukin 2 containing cell supernatants, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), or teleocidin B. Thus, anti-beta 2 appears to selectively activate T cells. In contrast to other T cell mitogens, anti-beta 2 microglobulin antibodies did not induce secondary immunoglobulin production in B cells from peripheral blood or spleen.
兔抗β2微球蛋白抗体已被描述为人类B淋巴细胞的一种强效有丝分裂原。然而，激活后进行分级分离时，只有富含T细胞的群体在积极分裂。纯化T细胞中增殖的诱导需要非T细胞的存在。已证明Daudi细胞（其细胞表面不表达β2微球蛋白）能产生“替代巨噬细胞因子”，支持抗β2诱导的T细胞分裂。通过添加含白细胞介素2的细胞上清液、12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）或teleocidin B，可以有效替代非T细胞。因此，抗β2似乎能选择性地激活T细胞。与其他T细胞有丝分裂原不同，抗β2微球蛋白抗体不会在外周血或脾脏的B细胞中诱导二次免疫球蛋白产生。
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