淋巴细胞激活中的细胞合作。II. 人外周血T淋巴细胞和B淋巴细胞对兔抗人β2-微球蛋白的协同反应。
Cellular cooperation in lymphocyte activation. II. Cooperative response of human peripheral T and B lymphocytes to rabbit anti-human beta 2-microglobulin.
作者信息
Kin K, Kasahara T, Itoh Y, Sakurabayashi I, Kawai T, Morita K
出版信息
Clin Exp Immunol. 1979 May;36(2):292-8.
In the present study we attempted to clarify the effects of anti-beta 2-microglobulin (a-beta 2m) on lymphocyte activation. Neither a-beta 2m IgG fraction nor F(ab')2 had a mitogenic effect on either highly purified T or B lymphocytes alone, while their mitogenic effect was observed when T and B lymphocytes were appropriately reconstituted. When T lymphocytes were reconstituted with mitomycin C (MMC) treated B lymphocytes, a negligible decrease in the response to a-beta 2m was observed compared to the response of an untreated mixture to a-beta 2m. On the other hand, when B lymphocytes were reconstituted with MMC-treated T lymphocytes, the response was markedly diminished. It was found, moreover, that the response of T lymphocytes separated by a semipermeable membrane from MMC-treated B lymphocytes was not enhanced, while a mixture of T and MMC-treated B lymphocytes in the same chamber showed a marked response. These results lead to the conclusion that the cells responding to a-beta 2m are mainly T lymphocytes whose response is strongly enhanced by B lymphocytes, and that for the mitogenic effect of a-beta 2m direct cell-to-cell interaction between T and B lymphocytes is necessary.
在本研究中，我们试图阐明抗β2-微球蛋白（a-β2m）对淋巴细胞激活的影响。单独的a-β2m IgG组分或F(ab')2对高度纯化的T淋巴细胞或B淋巴细胞均无促有丝分裂作用，而当T淋巴细胞和B淋巴细胞适当重组时，可观察到它们的促有丝分裂作用。当用丝裂霉素C（MMC）处理的B淋巴细胞重组T淋巴细胞时，与未处理混合物对a-β2m的反应相比，观察到对a-β2m的反应有可忽略不计的降低。另一方面，当用MMC处理的T淋巴细胞重组B淋巴细胞时，反应明显减弱。此外，发现通过半透膜与MMC处理的B淋巴细胞分离的T淋巴细胞的反应未增强，而在同一腔室中T淋巴细胞与MMC处理的B淋巴细胞的混合物显示出明显的反应。这些结果得出结论，对a-β2m有反应的细胞主要是T淋巴细胞，其反应被B淋巴细胞强烈增强，并且对于a-β2m的促有丝分裂作用，T淋巴细胞和B淋巴细胞之间的直接细胞间相互作用是必要的。
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