具有生物表面活性剂和抗菌活性的新型脂肽的发现与表征。你提供的原文中“produced by ”后面缺少具体内容，以上译文是根据现有内容尽量完善后给出的。

Discovery and characterization of novel lipopeptides produced by with biosurfactant and antimicrobial activities.

作者信息

Essghaier Badiaa, Naccache Chahnez, Ben-Miled Houda, Mottola Filomena, Ben-Mahrez Kamel, Mezghani Khemakhem Maha, Rocco Lucia

机构信息

Biochemistry and Biotechnology Laboratory LR01ES05, Faculty of Sciences of Tunis, University of Tunis Elmanar, 2092 Elmanar II, Tunisia.

Department of Environmental Biological and Pharmaceutical and Technologies (DiSTABiF), University of Campania L.Vanvitelli-Via Vivaldi, 43-81100 Caserta, Italy.

出版信息

3 Biotech. 2024 Nov;14(11):258. doi: 10.1007/s13205-024-04100-9. Epub 2024 Oct 4.

DOI:10.1007/s13205-024-04100-9

PMID:39372494

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11452367/

Abstract

UNLABELLED

The study aimed to evaluate the biosurfactants (BSs) production by SM-23 strain of identified by phenotypical and WGS analysis as . We first demonstrated the lipopeptides production by specie and studied their biochemical and molecular analysis as well as their biological potential. The GC-MS analysis indicated that methyl.2-hyroxydodecanoate was the major fatty acid compound with 33.22%. The maximum BSs production was obtained in LB medium supplemented by 1% olive oil (v/v) at 30 °C and 5% NaCl with 1.92 g/l. The obtained results revealed the significant biosurfactants/bioemulsifier potential compared to triton X100 with E24 of 100%, and an emulsification stability SE of 83%. The lipopeptides types were identified by FTIR analysis. A strong antimicrobial action was observed by the produced lipopeptides by the agar diffusion method against and The complete genome sequencing showed genes involved in the synthesis of multiple compounds identified as amphipathic cyclic lipopeptides such as locillomycin/locillomycin B/locillomycin C and bacillibactin. Our results highlighted significant lipopeptides properties displayed by that can be exploited to develop a novel strategy in the formulation of natural biocidal and fungicidal agents.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13205-024-04100-9.

摘要

未标记

本研究旨在评估经表型和全基因组测序分析鉴定为[具体菌种]的SM-23菌株产生生物表面活性剂（BSs）的情况。我们首先证明了[该菌种]产生脂肽，并研究了它们的生化和分子分析以及生物学潜力。气相色谱-质谱分析表明，2-羟基十二烷酸甲酯是主要脂肪酸化合物，含量为33.22%。在添加1%橄榄油（v/v）、30°C和5%氯化钠的LB培养基中，BSs产量最高，为1.92 g/l。所得结果表明，与曲拉通X100相比，该生物表面活性剂/生物乳化剂具有显著潜力，其乳化活性E24为100%，乳化稳定性SE为83%。通过傅里叶变换红外光谱分析鉴定了脂肽类型。通过琼脂扩散法观察到所产生的脂肽对[具体菌种1]和[具体菌种2]具有强烈的抗菌作用。全基因组测序显示，参与多种化合物合成的基因被鉴定为两亲性环状脂肽，如洛西洛霉素/洛西洛霉素B/洛西洛霉素C和杆菌铁载体。我们的结果突出了[该菌种]所显示的显著脂肽特性，可用于开发新型天然杀生物剂和杀真菌剂配方策略。