Rock G, Palmer D S
Thromb Haemost. 1985 Dec 17;54(4):780-3.
The level of Factor VIII procoagulant activity (VIIIC) was found to be more than 30% higher in heparinized plasma than in citrated plasma from the same donor when determined by a one-stage assay (1.34 U/ml versus 1.0 U/ml). However, after Al(OH)3 adsorption and two-stage assay, the VIIIC levels were not significantly different for the two types of plasma (0.90 +/- 0.25 U/ml versus 0.82 +/- 0.23 U/ml). If a one-stage assay was employed after adsorption, a similar low result was obtained (0.92 +/- 0.21 U/ml versus 0.91 +/- 0.12 U/ml). This indicated that the adsorption step itself was responsible for the difference in results. Parallel analyses of other VIII markers demonstrated that more VIIIC antigen (VIIICAg) was removed by adsorption of heparinized rather than citrated plasma (0.33 U/ml versus 0.12 U/ml) although there was no difference in the level of VIII-related antigen (VIIIRAg). The results show that adsorption of heparinized plasma prior to either one- or two-stage assay leads to a greater loss of both VIIIC and VIIICAg activity than observed with citrated plasma and indicate that, when applied to heparin plasma the two-stage assay for VIIIC underestimates the actual VIIIC content.
通过一步法检测发现，同一供体的肝素化血浆中凝血因子VIII促凝活性（VIIIC）水平比枸橼酸化血浆中的高30%以上（分别为1.34 U/ml和1.0 U/ml）。然而，经氢氧化铝吸附和两步法检测后，两种血浆的VIIIC水平无显著差异（分别为0.90±0.25 U/ml和0.82±0.23 U/ml）。如果吸附后采用一步法检测，也会得到类似的低结果（分别为0.92±0.21 U/ml和0.91±0.12 U/ml）。这表明吸附步骤本身导致了结果差异。对其他VIII标志物的平行分析表明，肝素化血浆吸附后去除的VIIIC抗原（VIIICAg）比枸橼酸化血浆更多（分别为0.33 U/ml和0.12 U/ml），尽管VIII相关抗原（VIIIRAg）水平无差异。结果表明，在一步法或两步法检测前对肝素化血浆进行吸附，导致VIIIC和VIIICAg活性的损失比枸橼酸化血浆更大，这表明，将两步法用于检测肝素化血浆中的VIIIC时，会低估实际的VIIIC含量。
