Stel H V, Veerman E C, Huisman J G, Janssen M C, van Mourik J A
Thromb Haemost. 1983 Dec 30;50(4):860-3.
A two-site immunoradiometric assay for factor VIII-procoagulant antigen (VIIICAg) that relies completely on monoclonal antibodies has been developed. By selecting an appropriate combination of these antibodies, it was possible to develop an assay in which the radiolabelled monoclonal antibody did not inhibit the binding of antigen to the solid-phase monoclonal antibodies. Thus, the entire test could be carried out as a one-step procedure. With this one-step assay, an amount of 0.0025 U VIIICAg/ml plasma could be detected after 4 hr of incubation, whereas 18 hr of incubation resulted in a lower limit of sensitivity of 0.0005 U VIIICAg/ml. The use of a one-step assay provides a significant advantage over the conventional two-step assay by simplifying, shortening and rendering the performance of the assay more convenient.
已开发出一种完全依赖单克隆抗体的用于凝血因子 VIII-促凝抗原（VIIICAg）的双位点免疫放射分析方法。通过选择这些抗体的适当组合，有可能开发出一种分析方法，其中放射性标记的单克隆抗体不会抑制抗原与固相单克隆抗体的结合。因此，整个测试可以作为一步法进行。采用这种一步法分析，孵育4小时后可检测到血浆中0.0025 U VIIICAg/ml的量，而孵育18小时则导致灵敏度下限为0.0005 U VIIICAg/ml。一步法分析的使用通过简化、缩短并使分析操作更方便，相对于传统的两步法分析具有显著优势。
