College of Mechanical and Vehicle Engineering, Hunan University, Changsha 410082, China.
Faculty of Engineering and Environment, Northumbria University, Newcastle upon Tyne NE1 8ST, United Kingdom.
ACS Sens. 2024 Oct 25;9(10):5570-5577. doi: 10.1021/acssensors.4c01949. Epub 2024 Oct 7.
Love-mode surface acoustic wave (SAW) sensors show great promise for biodetection applications owing to their low cost, digital output, and wireless passive capability, but their performance is often restricted by the availability of suitable sensitive membrane layers. Herein, a composite layer of electrospun fibers made from cellulose acetate and polyethylenimine, coated with gold nanoparticles, is proposed as a porous and sensitive membrane coated onto a love-mode SAW biosensor for monitoring gene sequences of . The results showed that the developed sensor exhibited an impressive sensitivity of 122.56 Hz/(nmol/L) for detecting gene sequences of , surpassing the sensitivity of conventional SAW sensors employing a bare Au film as the sensitive layer by 5-fold. The analysis revealed a remarkably linear detection (R of 0.97827) of gene sequences within the range of 0 to 100 nmol/L. The limit of detection was impressively low at 0.9116 nmol/L. The good stability and specificity of the biosensor in liquid environments were demonstrated for clinical diagnostics.
爱波表面声波（SAW）传感器由于其低成本、数字输出和无线无源能力，在生物检测应用中显示出巨大的潜力，但它们的性能通常受到合适敏感膜层的可用性的限制。在此，提出了一种由醋酸纤维素和聚乙烯亚胺制成的静电纺纤维复合层，涂有金纳米粒子，作为一种多孔敏感膜涂覆在爱波 SAW 生物传感器上，用于监测 基因序列。结果表明，开发的传感器对检测基因序列的灵敏度高达 122.56 Hz/（nmol/L），比传统的 SAW 传感器采用裸露的 Au 膜作为敏感层的灵敏度高 5 倍。分析表明，在 0 至 100 nmol/L 的范围内，基因序列的检测具有很好的线性（R2 为 0.97827）。检测限低至 0.9116 nmol/L。该生物传感器在液体环境中的良好稳定性和特异性已在临床诊断中得到证明。
