Buckner Eva A, Romero-Weaver Ana L, Schluep Sierra M, Bellamy Shawna K, Zimler Rebecca A, Kendziorski Natalie L, Ramirez Daviela, Whitehead Shelley A
Florida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Vero Beach, FL, USA.
J Med Entomol. 2025 Jan 13;62(1):146-154. doi: 10.1093/jme/tjae124.
Culex quinquefasciatus is an important mosquito vector responsible for the transmission of filarial worms, arthropod-borne viruses like Oropouche, St. Louis encephalitis, and West Nile and protozoans that cause avian malaria. Due to insecticide resistance documented in Cx. quinquefasciatus populations worldwide, integrated vector management programs can benefit from new strategies to control this species. The In2Care Mosquito Station (In2Care station), a commercially available dissemination station containing pyriproxyfen (PPF) and Beauveria bassiana spores, has been shown to be effective against skip-ovipositing Aedes aegypti and Aedes albopictus in previously conducted semifield and field trials. To determine the potential of Cx. quinquefasciatus adult females to autodisseminate PPF and if the In2Care station could be used for Cx. quinquefasciatus control, we assessed its efficacy in a semifield setting against wild Cx. quinquefasciatus. We found that the In2Care station was attractive to gravid Cx. quinquefasciatus females, with a significantly higher percentage of egg rafts laid in the In2Care station compared to alternative ovipots. Adult females successfully autodisseminated PPF from the In2Care station to surrounding ovipots, leading to a significant increase in mosquito emergence inhibition. Additionally, adult Cx. quinquefasciatus exposure to B. bassiana spores significantly reduced mosquito survivorship. These results suggest that the In2Care station may be effective against Cx. quinquefasciatus in addition to Ae. aegypti and Ae. albopictus. Additional field evaluations are needed to assess impacts at the population level.
致倦库蚊是一种重要的蚊虫媒介，可传播丝虫、奥罗普切病毒、圣路易斯脑炎病毒、西尼罗河病毒等节肢动物传播病毒以及引起禽疟的原生动物。由于全球范围内的致倦库蚊种群已出现杀虫剂抗性，综合媒介管理项目可受益于控制该物种的新策略。In2Care蚊虫诱捕站（In2Care诱捕站）是一种市售的传播站，含有吡丙醚（PPF）和球孢白僵菌孢子，在之前进行的半田间和田间试验中已证明对跳跃产卵的埃及伊蚊和白纹伊蚊有效。为了确定致倦库蚊成年雌蚊自动传播PPF的可能性以及In2Care诱捕站是否可用于控制致倦库蚊，我们在半田间环境中评估了其对野生致倦库蚊的功效。我们发现In2Care诱捕站对怀孕的致倦库蚊雌蚊具有吸引力，与其他产卵容器相比，在In2Care诱捕站产下的卵块百分比显著更高。成年雌蚊成功地将PPF从In2Care诱捕站自动传播到周围的产卵容器，导致蚊虫羽化抑制显著增加。此外，致倦库蚊成虫接触球孢白僵菌孢子显著降低了蚊虫的存活率。这些结果表明，In2Care诱捕站除了对埃及伊蚊和白纹伊蚊有效外，可能对致倦库蚊也有效。需要进行更多的田间评估以评估在种群水平上的影响。
