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一种使用戊二醛固定的人红细胞纯化抗A和抗B抗体的简单方法。

A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human red blood cells.

作者信息

Katsumata Y, Sato M

出版信息

Z Rechtsmed. 1985;95(3):163-6. doi: 10.1007/BF00201074.

DOI:10.1007/BF00201074
PMID:3937368
Abstract

A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human erythrocytes is described. Specific antibodies were first absorbed with the corresponding cells, then eluted by heating at 53 degrees-55 degrees C for 15 min. The method is simple and highly efficient with a fair recovery of 15.6%-34.4%.

摘要

描述了一种使用戊二醛固定的人红细胞纯化抗A和抗B抗体的简单方法。特异性抗体首先用相应细胞吸附，然后在53℃-55℃加热15分钟洗脱。该方法简单高效，回收率为15.6%-34.4%。

相似文献

1
A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human red blood cells.一种使用戊二醛固定的人红细胞纯化抗A和抗B抗体的简单方法。
Z Rechtsmed. 1985;95(3):163-6. doi: 10.1007/BF00201074.
2
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本文引用的文献

1
Affinity-purified Ulex europaeus lectin: preparation and serological characteristics.亲和纯化的荆豆凝集素：制备及血清学特性
J Forensic Sci Soc. 1983 Jul;23(3):225-31. doi: 10.1016/s0015-7368(83)72247-4.
2
[Studies on the purification of anti-A allohemagglutinin by affinity chromatography. (1) Comparative efficiency of several eluting methods].[亲和层析法纯化抗A同种血凝素的研究。(1) 几种洗脱方法的比较效率]
Nihon Hoigaku Zasshi. 1984 Feb;38(1):49-58.
3
[Immunoadsorbents for the purification of MN blood group system antibodies].
用于纯化MN血型系统抗体的免疫吸附剂
Nihon Hoigaku Zasshi. 1983 Apr;37(2):88-94.
4
Studies on human antibodies. IV. Purification and properties of anti-A and anti-B obtained by absorption and elution from insoluble blood group substances.人类抗体研究。IV. 通过从不溶性血型物质中吸收和洗脱获得的抗A和抗B的纯化及特性
J Exp Med. 1966 Jun 1;123(6):1061-81. doi: 10.1084/jem.123.6.1061.