一种使用戊二醛固定的人红细胞纯化抗A和抗B抗体的简单方法。
A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human red blood cells.
作者信息
Katsumata Y, Sato M
出版信息
Z Rechtsmed. 1985;95(3):163-6. doi: 10.1007/BF00201074.
PMID:3937368
Abstract
A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human erythrocytes is described. Specific antibodies were first absorbed with the corresponding cells, then eluted by heating at 53 degrees-55 degrees C for 15 min. The method is simple and highly efficient with a fair recovery of 15.6%-34.4%.
摘要
描述了一种使用戊二醛固定的人红细胞纯化抗A和抗B抗体的简单方法。特异性抗体首先用相应细胞吸附，然后在53℃-55℃加热15分钟洗脱。该方法简单高效，回收率为15.6%-34.4%。
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