Feng Ke-Ping, Liu Ji-Mei, Chen Ri-Dao, Fei Qiao-Man, Xie Ke-Bo, Chen Da-Wei, Dai Jun-Gui
National-Local Joint Engineering Research Center of Biodiagnostic & Biotherapy, Department of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an710004, China.
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, CAMS Key Laboratory of Enzyme and Biocatalysis of Natural Drugs; and NHC Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing100050, China.
J Asian Nat Prod Res. 2025 Apr;27(4):568-576. doi: 10.1080/10286020.2024.2411710. Epub 2024 Oct 7.
is widely used in traditional Chinese medicine and contains rich bioactive compounds. These compounds often have a methyl group at their 4'-OH position catalyzed by methyltransferases. Therefore, studying methyltransferases in plants is of great significance. In this study, a flavonol methyltransferase, EpOMT4, was isolated from B.L. Guo. The recombinant enzyme regiospecifically transferred a methyl group to the 4'-OH position of 8-prenylkaempferol forming icaritin. The study demonstrates that enzymatic methylation of flavonoids in plants holds significant potential and could provide a promising alternative method for the biosynthetic production of bioactive methylated prenylflavonoids.
它在传统中药中被广泛使用，并且含有丰富的生物活性化合物。这些化合物在甲基转移酶的催化下，其4'-OH位置通常具有一个甲基基团。因此，研究植物中的甲基转移酶具有重要意义。在本研究中，从郭宝林（B.L. Guo）中分离出一种黄酮醇甲基转移酶EpOMT4。该重组酶区域特异性地将一个甲基基团转移到8-异戊烯基山奈酚的4'-OH位置，形成淫羊藿素。该研究表明，植物中黄酮类化合物的酶促甲基化具有巨大潜力，并且可以为生物活性甲基化异戊烯基黄酮类化合物的生物合成生产提供一种有前景的替代方法。
