Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
J Photochem Photobiol B. 2019 Aug;197:111550. doi: 10.1016/j.jphotobiol.2019.111550. Epub 2019 Jul 5.
The plant species of the genus Epimedium L. are well-known traditional Chinese medicinal herbs with special therapeutic effects on human beings and animals in invigorating sexuality and strengthening muscles and bones. In large-scale cultivating Epimedium that is a typical shade plant species, they are arbitrarily covered with black colored shade nets. However, their optimal growth conditions, especially light, are still less understood. During the investigation of different light qualities on the growth of Epimedium pseudowushanense, it was found that, all the values of plant growth characteristics (except shoot number) and photosynthetic characteristics were lower under red, yellow, or blue light treatment than under white light treatment. However, yellow light treatment had beneficial effects on shoot number, dry biomass (per plant) as well as net photosynthesis rate (Pn) and maximal apparent quantum efficiency (AQY) in E. pseudowushanense when compared with red or blue light treatment. More importantly, we found that E. pseudowushanense accumulated higher levels of bioactive flavonoids under yellow light treatment than under white, red, or blue light treatment. Furthermore, both RNAseq and qPCR analyses revealed that yellow light could highly up-regulate the expression levels of flavonoid biosynthetic genes, in particular CHS1, F3H1, PT_5, and raGT_5 that possibly contributed to the enhanced accumulation of bioactive flavonoids in E. pseudowushanense. Taken together, our study revealed that yellow light is the optimal light for the growth of E. pseudowushanense. Our results provided key information on how to improve the cultivation condition and concurrently enhance the accumulation of bioactive flavonoids in E. pseudowushanense.
植物物种的淫羊藿属是著名的传统中药对人类和动物有特殊的治疗效果在壮阳和强筋健骨。在大规模种植淫羊藿，是一个典型的耐荫植物物种，他们任意覆盖黑色遮光网。然而，他们的最佳生长条件，尤其是光，仍然不太了解。在不同的光质对黄皮淫羊藿生长的影响的调查中，发现，除了芽数外，所有植物生长特性（plant growth characteristics）和光合特性（photosynthetic characteristics）的值在红光、黄光或蓝光处理下都低于白光处理。然而，与红光或蓝光处理相比，黄光处理对黄皮淫羊藿的芽数、单株干生物量（dry biomass per plant）、净光合速率（Pn）和最大表观量子效率（AQY）有有益的影响。更重要的是，我们发现黄皮淫羊藿在黄光处理下积累的生物活性类黄酮水平高于白光、红光或蓝光处理。此外，RNAseq 和 qPCR 分析表明，黄光可以高度上调类黄酮生物合成基因的表达水平，特别是 CHS1、F3H1、PT_5 和 raGT_5，这可能有助于黄皮淫羊藿中生物活性类黄酮的积累。总之，我们的研究表明，黄光是黄皮淫羊藿生长的最佳光线。我们的研究结果提供了如何改善栽培条件并同时提高黄皮淫羊藿中生物活性类黄酮积累的关键信息。
