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sp. nov.,从牛和野猪中分离得到。

sp. nov., isolated from cattle and feral swine.

机构信息

Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, USA.

Bioproducts Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, USA.

出版信息

Int J Syst Evol Microbiol. 2024 Oct;74(10). doi: 10.1099/ijsem.0.006524.

Abstract

Nine strains were isolated from cattle and feral swine faeces: three were recovered during a 2007 -associated outbreak linked to a dairy, and the other six were isolated during a 2009-2010 survey of farms and ranches in Central California. The species identification of these strains could not be determined by 16S rRNA gene sequencing but were most similar to and . Additional typing indicated that the nine strains composed a discrete novel clade related to and . A polyphasic study was undertaken here to clarify their taxonomic position. Phylogenetic analyses were performed based on 16S rRNA gene sequences and the concatenated sequences of 330 core genes. The core gene analysis placed the nine strains into a clade well separated from the other taxa, indicating that these strains represent a novel species. Pairwise digital DNA-DNA hybridization and average nucleotide identity values between these strains and other campylobacters are lower than 16 and 73%, respectively, further supporting their placement into a novel taxon. Standard phenotypic testing was performed. All strains are microaerobic or anaerobic, motile, Gram-negative, slightly-curved rods that are oxidase positive but catalase negative. Strains can be distinguished from the other catalase-negative species using phenotypic markers such as motility, oxidase activity, cephalothin resistance, hippuricase activity, growth at 30 °C, and α-haemolysis. The data presented here show that these strains represent a novel species within , for which the name sp. nov. (type strain RM6914=LMG 32304=CCUG 75329) is proposed.

摘要

从牛和野猪粪便中分离出九株菌株

三株是在 2007 年与一家奶牛场相关的暴发中分离出来的,另外六株是在 2009 年至 2010 年对加利福尼亚中部农场和牧场的调查中分离出来的。这些菌株的种属鉴定不能通过 16S rRNA 基因测序确定,但与 和 最为相似。进一步的 分型表明,这九株菌株构成了一个与 和 相关的独特新型分支。在这里进行了多相研究以阐明它们的分类地位。基于 16S rRNA 基因序列和 330 个核心基因的串联序列进行了系统发育分析。核心基因分析将这九株菌株置于与其他 种属明显分离的分支中,表明这些菌株代表了一个新的 种属。这些菌株与其他弯曲菌属之间的两两数字 DNA-DNA 杂交和平均核苷酸同一性值均低于 16%和 73%,进一步支持它们归入一个新的分类群。进行了标准的表型测试。所有菌株均为微需氧或厌氧、运动、革兰氏阴性、轻度弯曲的棒状,氧化酶阳性但过氧化氢酶阴性。菌株可以通过运动性、氧化酶活性、头孢噻吩抗性、马尿酸酶活性、30°C 生长和 α-溶血等表型标记与其他过氧化氢酶阴性的 种属区分开来。本文介绍的这些数据表明,这些菌株代表了 属内的一个新种,建议将其命名为 sp. nov.(模式株 RM6914=LMG 32304=CCUG 75329)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4168/11457942/8ea7585ff6a7/ijsem-74-06524-g001.jpg

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