Lynch Caoimhe, Peeters Charlotte, Walsh Niamh, McCarthy Conor, Coffey Aidan, Lucey Brigid, Vandamme Peter
Department of Biological Sciences, Munster Technological University, Rossa Ave, Bishopstown Cork T12 P928, Ireland.
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005510.
Strains LMG 7974 and LMG 8286 represent single, novel lineages with and as nearest phylogenomic neighbours, respectively. The results of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses of LMG 7974, LMG 8286 and type strains of species of the genus confirmed that these strains represent novel species of the genus . The 16S rRNA gene sequences of both strains showed highest identity towards (97.84 and 98.74 %, respectively). Strains LMG 7974 and LMG 8286 shared 72.5 and 73.7% ANI, respectively, with their nearest phylogenomic neighbours and less than 21 % dDDH. The draft genome sizes of LMG 7974 and LMG 8286 are 1 945429 bp and 1 708214 bp in length with percentage DNA G+C contents of 33.8 and 37.2 %, respectively. Anomalous biochemical characteristics and low MALDI-TOF mass spectrometry log scores supported their designation as representing novel species of the genus . We therefore propose to classify strain LMG 7974 (=CCUG 20705) as the type strain of the novel species sp. nov. and strain LMG 8286 (=CCUG 24193, NCTC 11879) as the type strain of the novel species sp. nov.
菌株LMG 7974和LMG 8286分别代表单一的新谱系,其最近的系统基因组邻居分别是[此处原文缺失相关信息]和[此处原文缺失相关信息]。对LMG 7974、LMG 8286以及[此处原文缺失相关属名]属物种的模式菌株进行的平均核苷酸同一性(ANI)和数字DNA-DNA杂交(dDDH)分析结果证实,这些菌株代表[此处原文缺失相关属名]属的新物种。这两个菌株的16S rRNA基因序列与[此处原文缺失相关物种名]的同一性最高(分别为97.84%和98.74%)。菌株LMG 7974和LMG 8286与其最近的系统基因组邻居的ANI分别为72.5%和73.7%,dDDH小于21%。LMG 7974和LMG 8286的基因组草图大小分别为1945429 bp和1708214 bp,DNA G+C含量百分比分别为33.8%和37.2%。异常的生化特征和较低的基质辅助激光解吸电离飞行时间质谱对数得分支持将它们指定为代表[此处原文缺失相关属名]属的新物种。因此,我们建议将菌株LMG 7974(=CCUG 20705)分类为新物种[此处原文缺失新物种名] sp. nov.的模式菌株,将菌株LMG 8286(=CCUG 24193,NCTC 11879)分类为新物种[此处原文缺失新物种名] sp. nov.的模式菌株。