微流控技术在适体 SELEX 和适体传感器中的设计与应用。
Design and application of microfluidics in aptamer SELEX and Aptasensors.
机构信息
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.
出版信息
Biotechnol Adv. 2024 Dec;77:108461. doi: 10.1016/j.biotechadv.2024.108461. Epub 2024 Oct 5.
Aptamers are excellent recognition molecules obtained from systematic evolution of ligands by exponential enrichment (SELEX) that have been extensively researched for constructing aptasensors. However, in the process from SELEX to the construction of aptasensors, there are many disadvantages, such as tedious and repetitive operations, interference from external factors, and low efficiency, which seriously limits their application scope and development. Introducing the microfluidic technology can realize the integration and intelligence of SELEX and aptasensing, improve the efficiency of SELEX, and enhance the detection performance and convenience of aptasensing. Hence, in this review, the characteristics of various chips based on different driving forces are described firstly. And then summarizing the design of microfluidic devices based on different SELEX methods and showing the strategies of microfluidic aptasensors based on different detection modes. Finally, discussing the difficulties and challenges encountered when microfluidic is integrated with the SELEX and the aptasensors.
适体是通过指数富集配体系统进化（SELEX）获得的优秀识别分子，已广泛用于构建适体传感器。然而，在从 SELEX 到适体传感器构建的过程中，存在许多缺点，例如操作繁琐重复、受外部因素干扰以及效率低下，这严重限制了它们的应用范围和发展。引入微流控技术可以实现 SELEX 和适体传感的集成和智能化，提高 SELEX 的效率，并增强适体传感的检测性能和便利性。因此，在这篇综述中，首先描述了基于不同驱动力的各种芯片的特点。然后总结了基于不同 SELEX 方法的微流控装置的设计，并展示了基于不同检测模式的微流控适体传感器的策略。最后，讨论了微流控与 SELEX 和适体传感器集成时遇到的困难和挑战。
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