Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow, G4 0RE, United Kingdom.
Department of Biotechnology, Nigeria Defence Academy, PMB 2109, Kaduna, Kaduna State, Nigeria.
Exp Parasitol. 2024 Nov;266:108844. doi: 10.1016/j.exppara.2024.108844. Epub 2024 Oct 5.
Ten compounds, six extracts and five fractions obtained from three Nigerian plants were assayed for their in vitro antitrypanosomal and antileishmanial activities. Each plant was extacted with hexane, ethyl acetate, and methanol. Isolated compounds were characterized and identified based on their NMR chemical shifts and comparison to literature reports. The crude extracts, fractions and isolated compounds were tested against the kinetoplastid parasites: bloodstream forms of Trypanosoma brucei Lister 427WT and the derived multi-drug resistant clone B48, and promastigote forms of Leishmania mexicana cas9/T7 and the derived clone cas9ΔNT1. Column chromatography of the extracts using silica gel yielded ten compounds identified as curzerenone, epi-curzerenone, chloranthene F, isofuranodienone, 8(17)-12E-labdadiene-15, 16-dial and 15-hydroxy-8(17),12E-labdadiene-16-al from Siphonochilus aetiopicus, lupeol, linalolic acid and spinasterone from Calliandra portoricensis, and abruquinone B from Abrus precatorius. The assay results showed that the Siphonochilus aetiopicus and Calliandra portoricensis crude extracts, fractions and compounds displayed moderate activity against the Trypanosoma brucei but showed less activity against Leishmania mexicana. Abrus precatorius crude extract, fraction, and isolated compound exhibited only weak trypanocidal and leishmanicidal activities against both kinetoplastid parasites tested. These findings have provided evidence for the use of Siphonochilus aetiopicus and Calliandra portoricensis in traditional medicine relating to parasitic diseases.
从三种尼日利亚植物中分离得到的十种化合物、六种提取物和五种馏分，用于体外抗锥虫和抗利什曼原虫活性的测定。每种植物都用正己烷、乙酸乙酯和甲醇提取。根据其 NMR 化学位移和与文献报道的比较，对分离得到的化合物进行了特征和鉴定。粗提取物、馏分和分离得到的化合物均针对原生动物寄生虫进行了测试：锥虫的血腔形式 Trypanosoma brucei Lister 427WT 和由此产生的多药耐药克隆 B48，以及利什曼原虫的前鞭毛体形式 Leishmania mexicana cas9/T7 和由此产生的克隆 cas9ΔNT1。使用硅胶对提取物进行柱色谱分离，得到十种化合物，分别鉴定为莪术酮、表莪术酮、氯蒽酮 F、异呋喃二烯酮、8(17)-12E-拉伯达二烯-15,16-二醛和 15-羟基-8(17),12E-拉伯达二烯-16-醇，从 Siphonochilus aetiopicus 中分离得到；从 Calliandra portoricensis 中分离得到羽扇豆醇、亚麻酸和 spinasterone；从 Abrus precatorius 中分离得到 abruquinone B。测定结果表明，Siphonochilus aetiopicus 和 Calliandra portoricensis 粗提取物、馏分和化合物对 Trypanosoma brucei 具有中等活性，但对 Leishmania mexicana 的活性较低。Abrus precatorius 粗提取物、馏分和分离得到的化合物对两种测试的原生动物寄生虫仅有微弱的杀锥虫和杀利什曼原虫活性。这些发现为 Siphonochilus aetiopicus 和 Calliandra portoricensis 在与寄生虫病相关的传统医学中的应用提供了证据。
