Bompart G, Clamens S
INSERM U 113-Faculté de Médecine, Toulouse.
J Toxicol Clin Exp. 1990 Jan-Feb;10(1):3-14.
In vitro, the addition of various concentrations of sodium selenite (Na2SeO3, 5H2O) to liver and lung microsomal fractions of rats previously treated with 3-methylcholanthrene shows a decrease in the different benzo(a)pyrene (B(a)P) metabolites. This inhibition varies, depending on the metabolite considered and in relation to both the selenium level and the origin of the microsomal suspension. In vivo, on rats treated with B(a)P or B(a)P+ Na2SeO3, the results show a high aryl hydrocarbon hydroxylase (A.H.H) activity in lung microsomes; however selenium does not exhibit any effect. The slight increase of lung cytochrome P450 levels is not significant. Moreover, B(a)P and B(a)P+ Na2SeO3 do not induce any effect on liver A.H.H activity and cytochrome P450 levels. The insufficient Se concentration in the tissues could explain the lack of in vivo effect.
在体外实验中，向先前用3 - 甲基胆蒽处理过的大鼠肝脏和肺微粒体组分中添加不同浓度的亚硒酸钠（Na2SeO3·5H2O），结果显示不同的苯并(a)芘（B(a)P）代谢产物减少。这种抑制作用因所考虑的代谢产物以及硒水平和微粒体悬浮液的来源而异。在体内实验中，对用B(a)P或B(a)P + Na2SeO3处理的大鼠而言，结果显示肺微粒体中具有较高的芳烃羟化酶（A.H.H）活性；然而，硒未表现出任何作用。肺细胞色素P450水平的轻微升高并不显著。此外，B(a)P和B(a)P + Na2SeO3对肝脏A.H.H活性和细胞色素P450水平均未产生任何影响。组织中硒浓度不足可能解释了体内无作用的现象。
