Department of Microbiology, School of Life Sciences, Central University of Rajasthan, Ajmer, 305817, India.
Department of Biostatistics and Bioinformatics, ICMR-National Institute for Research in Environmental Health, Bhopal, 462030, India.
Lett Appl Microbiol. 2024 Oct 1;77(10). doi: 10.1093/lambio/ovae095.
Antimicrobial resistance (AMR) has become one of the most serious threats to One Health. Aquatic environments are an ideal non-clinical AMR reservoir and can act as a key battlefront for tackling the AMR. However, AMR data using the One Health approach remain scarce in aquatic environments worldwide. Here, we extensively assessed AMR in Escherichia coli isolated from urban and rural lake ecosystems using the One Health perspective. A total of 162 E. coli isolates obtained from lakes were tested against 25 antimicrobials using an in-vitro antimicrobial susceptibility testing method. A low (2%) to moderate (45%) drug resistance rate was found for all antimicrobials used in human/veterinary medicine or animal/plant agriculture. However, <80% E. coli isolates exhibited multidrug resistance (MDR) phenotype to highly important (amikacin, gentamicin, trimethoprim) or critically important (amoxicillin, ampicillin, colistin) drugs of both human and veterinary medicine. Of concern, >50% of E. coli isolates exhibited MDR to drugs used as last-resorts (chloramphenicol, colistin) or as frontline (nitrofurantoin, sulfamethoxazole, ampicillin, gentamicin) against E. coli infections. In conclusion, the presence of MDR E. coli strains in urban or rural lake ecosystems highlights their possible role as AMR reservoirs with potential One Health risks.
抗微生物药物耐药性（AMR）已成为对“同一健康”构成的最严重威胁之一。水生环境是一个理想的非临床 AMR 储存库，可作为应对 AMR 的关键前沿阵地。然而，在全球范围内，采用“同一健康”方法的水生环境中的 AMR 数据仍然很少。在这里，我们从“同一健康”的角度广泛评估了城市和农村湖泊生态系统中分离出的大肠杆菌中的 AMR。使用体外药敏试验方法，对从湖泊中获得的总共 162 株大肠杆菌分离株进行了 25 种抗生素的药敏试验。发现所有用于人类/兽医医学或动物/植物农业的抗生素的耐药率较低（2%）至中度（45%）。然而，<80%的大肠杆菌分离株对人类和兽医医学中高度重要（阿米卡星、庆大霉素、甲氧苄啶）或极重要（阿莫西林、氨苄西林、黏菌素）的药物表现出多药耐药（MDR）表型。值得关注的是，超过 50%的大肠杆菌分离株对用作最后手段（氯霉素、黏菌素）或一线药物（呋喃妥因、磺胺甲恶唑、氨苄西林、庆大霉素）的药物表现出 MDR，用于治疗大肠杆菌感染。总之，城市或农村湖泊生态系统中存在 MDR 大肠杆菌菌株，突出了它们作为 AMR 储存库的可能作用，存在潜在的“同一健康”风险。
