埃塞俄比亚中部安博及其周边地区 backyard chicken 分离出的大肠杆菌的鉴定及抗菌药敏谱
Identification and antimicrobial susceptibility profile of Escherichia coli isolated from backyard chicken in and around ambo, Central Ethiopia.
作者信息
Sarba Edilu Jorga, Kelbesa Kebede Abdisa, Bayu Morka Dandecha, Gebremedhin Endrias Zewdu, Borena Bizunesh Mideksa, Teshale Ayichew
机构信息
Department of Veterinary Sciences, Ambo University, P O Box 19, Ambo, Ethiopia.
Department of Veterinary Laboratory Technology, Ambo University, P O Box 19, Ambo, Ethiopia.
出版信息
BMC Vet Res. 2019 Mar 11;15(1):85. doi: 10.1186/s12917-019-1830-z.
BACKGROUND
Escherichia coli is bacteria that exist as commensal in the intestine of animals and humans, but pathogenic strains cause disease in chickens. The development of antimicrobial resistance in E. coli is one of major concern worldwide. A cross-sectional study was conducted from November, 2015 to April, 2016 in and around Ambo town on backyard chicken with the objectives of isolating E. coli from selected visceral organs, assessment of potential risk factor and determination of antimicrobial resistance pattern of the isolates.
RESULTS
The overall isolation rate of E. coli was 11.5% (80/694) [95% CI: 9.64-14.61] and 32.5% (62/191) [95% CI: 25.39-39.09] at organ and chicken level, respectively. E. coli isolation rate was 15.2% (29/191), 13.6% (27/191), 6.3% (12/191) and 10.7% (13/121) from spleen, liver, kidney and ovary samples, respectively. The multivariable logistic regression analysis revealed higher probability of E. coli isolation from adult (adjusted Odds ratio [aOR] =2.5, P = 0.013) than younger chickens, from clinically sick chickens (aOR = 3.0, P = 0.003) than apparently healthy. E. coli isolates were 100% susceptible to ciprofloxacin, norfloxacin and sulfamethoxazole-trimethoprim followed by 89-63.4% susceptibility to gentamicin, streptomycin, ceftazidime, nalidxic acid, nitrofurantoin, kanamycin, amikacin and chloramphenicol. Whereas, 100% resistance was observed against cloxacilin, cefotaxime and amoxicillin, whereas 92.7 and 46.3% were resistant to cefuroxime, and tetracycline, respectively. Multidrug resistant (MDR) was observed in 78.1% (64/82) of the isolates which exhibited 5 different MDR patterns to 7 antimicrobial classes.
CONCLUSIONS
Higher isolation rate of E. coli was observed from visceral organs of chickens. Age and health status were predictors of E. coli isolation. Remarkable numbers of the isolates are resistant to different antimicrobials and multidrug resistant E coli isolates are widespread in the area.
背景
大肠杆菌是存在于动物和人类肠道中的共生菌，但致病性菌株会导致鸡发病。大肠杆菌中抗菌药物耐药性的发展是全球主要关注的问题之一。2015年11月至2016年4月在安博镇及其周边地区对后院鸡进行了一项横断面研究，目的是从选定的内脏器官中分离大肠杆菌，评估潜在风险因素并确定分离株的抗菌药物耐药模式。
结果
大肠杆菌在器官和鸡水平的总体分离率分别为11.5%（80/694）[95%可信区间：9.64 - 14.61]和32.5%（62/191）[95%可信区间：25.39 - 39.09]。从脾脏、肝脏、肾脏和卵巢样本中分离大肠杆菌的比率分别为15.2%（29/191）、13.6%（27/191）、6.3%（12/191）和10.7%（13/121）。多变量逻辑回归分析显示，从成年鸡分离大肠杆菌的概率更高（调整优势比[aOR]=2.5，P = 0.013），高于幼鸡；从临床患病鸡分离的概率更高（aOR = 3.0，P = 0.003），高于明显健康的鸡。大肠杆菌分离株对环丙沙星、诺氟沙星和磺胺甲恶唑 - 甲氧苄啶100%敏感，其次对庆大霉素、链霉素、头孢他啶、萘啶酸、呋喃妥因、卡那霉素、阿米卡星和氯霉素的敏感性为89% - 63.4%。然而，观察到对氯唑西林、头孢噻肟和阿莫西林100%耐药，而对头孢呋辛和四环素的耐药率分别为92.7%和46.3%。在78.1%（64/82）的分离株中观察到多重耐药（MDR），这些分离株对7类抗菌药物表现出5种不同的MDR模式。
结论
从鸡的内脏器官中观察到较高的大肠杆菌分离率。年龄和健康状况是大肠杆菌分离的预测因素。大量分离株对不同抗菌药物耐药，并且多重耐药大肠杆菌分离株在该地区广泛存在。
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