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使用罗技光学显微镜系统对钙化组织制备磨片的各种塑料包埋方法进行评估。

An evaluation of various plastic embedding methods for preparing ground sections from calcified tissue with the Logitech system for light microscopy.

作者信息

Wong F T

出版信息

Calcif Tissue Int. 1985 Dec;37(6):669-72. doi: 10.1007/BF02554928.

Abstract

Three plastic processing methods for preparing ground sections from hard tissue applicable to light microscopy are reviewed. The requirement for production of high quality ground sections of tooth and bone for pathological diagnosis is discussed and a new machine system technology, namely the LP 30 Lapping Machine (Logitech Ltd., Dunbartonshire, Scotland) which meets these requirements is described. The new approach is justified by the results, since sections of quality are produced with increased efficiency, while the operator's working conditions are distinctly improved. Ground sections, 10-20 microns, are being produced from glycol methacrylate impregnated specimens with the employment of the Leitz diamond saw and the Logitech section grounding system. Good GMA ground sections form an invaluable aid to microscopic diagnosis of many disorders of enamel as well as metabolic bone diseases.

摘要

本文综述了三种适用于光学显微镜观察的硬组织磨片制备的塑料加工方法。讨论了制备高质量牙齿和骨骼病理诊断磨片的要求,并介绍了一种满足这些要求的新型机器系统技术,即LP 30研磨机(罗技有限公司,苏格兰邓巴顿郡)。新方法的结果证明是合理的,因为它提高了磨片质量和生产效率,同时显著改善了操作人员的工作条件。使用徕卡钻石锯和罗技磨片制备系统,从甲基丙烯酸乙二醇酯浸渍的标本中制备出了10-20微米的磨片。良好的甲基丙烯酸乙二醇酯磨片对许多牙釉质疾病和代谢性骨病的显微镜诊断具有重要价值。

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