Neonatal Hearing Screening Associated with Congenital Syphilis and HIV.

: Hearing is fundamental for the development of newborns. The Universal Neonatal Hearing Screening (UNHS) in Brazil seeks to detect hearing loss early to minimize the impact on the child's hearing health. Some coping with underreporting of Sexually Transmitted Infections (STIs), such as congenital syphilis and HIV, are important, considering these situations are correlated with neonatal hearing loss. : To assess the prevalence of congenital syphilis and HIV in newborns from two maternity hospitals in Greater Florianópolis and the impact on hearing, based on the results of the UNHS retest from 2018 to 2022. : Retrospective cross-sectional study with secondary data of newborns treated at a SUS Hearing Health Care Service (SASA) between 2018 and 2022, referred for OAE and BAEP retests. : Of the 1211 newborns referred for retesting, 283 presented the investigated infections. Of these, 170 were diagnosed with congenital syphilis (59.86%), 105 were diagnosed with HIV (36.67%), and 8 with both infections (2.82%). When performing the retest, one newborn did not present a satisfactory auditory result, and the others passed when the OAE and BAEP tests were observed. : UNHS is effective, and retests are fundamental in the screening and proper monitoring of the hearing health of newborns. Public health guidelines are evidenced, contributing to neonatal health and hearing health screening.