Zoet Yvonne M, Heidt Sebastiaan, van der Linden-van Oevelen Marissa J H, Haasnoot Geert W, Claas Frans H J
Immunology-ETRL, Leiden University Medical Center (LUMC), Leiden, Netherlands.
Eurotransplant, Leiden, Netherlands.
Front Genet. 2024 Sep 23;15:1451748. doi: 10.3389/fgene.2024.1451748. eCollection 2024.
Eurotransplant is responsible for the international allocation of organs between eight countries in Europe. All HLA laboratories affiliated to Eurotransplant must be EFI or ASHI-accredited and must participate in the Eurotransplant external proficiency testing (EPT) program, organized by the Eurotransplant Reference Laboratory (ETRL). EPT within Eurotransplant has a long tradition, starting in 1978. The current EPT program consists of the following schemes: HLA typing including serology, CDC crossmatching, HLA-specific antibody detection, and identification. Participants enter the results of laboratory tests using a web-based application. Assessed results are visible on the website. An additional component called "patient-based cases" runs since 2016. Results are summarized and published on the EPT website. Furthermore, these results are discussed during the annual extramural tissue typers meeting, which is organized by the ETRL. Thanks to this EPT program, the performance of all HLA laboratories affiliated to Eurotransplant can be monitored and corrected, if necessary. Because all affiliated laboratories are assessed in the same EPT program, where these laboratories show to be consistent in most of their results, Eurotransplant EPT has proven to be an efficient tool to create a more uniform level of quality of histocompatibility testing within Eurotransplant.
欧洲器官移植组织负责欧洲八个国家之间器官的国际分配。所有隶属于欧洲器官移植组织的HLA实验室都必须获得欧洲免疫遗传学学会（EFI）或美国组织相容性与免疫学会（ASHI）的认可，并且必须参加由欧洲器官移植参考实验室（ETRL）组织的欧洲器官移植组织外部能力验证（EPT）计划。欧洲器官移植组织内部的EPT有着悠久的传统，始于1978年。当前的EPT计划包括以下方案：HLA分型，包括血清学、补体依赖细胞毒交叉配型、HLA特异性抗体检测和鉴定。参与者使用基于网络的应用程序输入实验室检测结果。评估结果可在网站上查看。自2016年起运行一个名为“基于患者的病例”的附加组件。结果会进行汇总并在EPT网站上公布。此外，这些结果会在由ETRL组织的年度校外组织分型专家会议上进行讨论。多亏了这个EPT计划，如有必要，欧洲器官移植组织下属所有HLA实验室的表现都能得到监测和纠正。由于所有附属实验室都在同一个EPT计划中接受评估，这些实验室在大多数结果上表现一致，欧洲器官移植组织的EPT已被证明是一个有效的工具，能够在欧洲器官移植组织内创建更统一的组织相容性检测质量水平。
