Mokrzyński Krystian, Szewczyk Grzegorz
Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
Photochem Photobiol. 2025 May-Jun;101(3):546-549. doi: 10.1111/php.14030. Epub 2024 Oct 8.
Rose bengal (RB) is a widely used photosensitizer for determining quantum yields of singlet oxygen generation. While it is known to aggregate in polar environments at concentrations above 2 μM, the relationship between RB concentration and singlet oxygen photogeneration remains unclear. This study investigates the shift from monomeric to dimeric RB with increasing concentration and its impact on singlet oxygen generation in DO-based solutions and DMPC liposomes. Absorbance maxima for RB were observed at 514 nm (dimer) and 549 nm (monomer), with ionic environments influencing aggregation rates. Singlet oxygen phosphorescence showed non-linear dependency above 2 μM, indicating the effects of aggregation. Results suggest that RB concentrations should be kept at 1 μM or lower in photochemical studies to avoid aggregation-related discrepancies in singlet oxygen yield determination. These findings highlight the importance of considering RB aggregation in photochemical research and medical applications.
孟加拉玫瑰红（RB）是一种广泛用于测定单线态氧生成量子产率的光敏剂。虽然已知其在浓度高于2μM时会在极性环境中聚集，但RB浓度与单线态氧光生成之间的关系仍不清楚。本研究调查了随着浓度增加，RB从单体向二聚体的转变及其对基于DO的溶液和DMPC脂质体中单线态氧生成的影响。RB的最大吸收峰在514nm（二聚体）和549nm（单体）处观察到，离子环境会影响聚集速率。单线态氧磷光在2μM以上表现出非线性依赖性，表明存在聚集效应。结果表明，在光化学研究中，RB浓度应保持在1μM或更低，以避免在单线态氧产率测定中出现与聚集相关的差异。这些发现突出了在光化学研究和医学应用中考虑RB聚集的重要性。
