Chun P K, Sensabaugh G F, Vedros N A
J Gen Microbiol. 1985 Nov;131(11):3105-15. doi: 10.1099/00221287-131-11-3105.
The electrophoretic mobilities of 12 enzymes from 19 Neisseria species (including 6 strains of N. perflava), Gemella haemolysans, Escherichia coli and Branhamella catarrhalis were characterized by polyacrylamide slab gel electrophoresis. All strains and species tested exhibited qualitatively different zymogram patterns. Species and strain relationships were quantified by pairwise comparisons of all 12 enzyme systems to obtain similarity indices; these data were subjected to numerical clustering methods to obtain groups and a phenogram. The electrophoretic classification compared favourably with those obtained by other criteria. In addition, the quantitative clustering data indicated that N. ovis and N. caviae are sufficiently different from the other Neisseria species to warrant their separation into a distinct group. These two species also lacked the characteristic NADPH-diaphorase zymogram pattern found in all the other Neisseria species. Intra-species similarity indices were generally greater than the inter-species index values. However, certain species such as N. meningitidis and N. gonorrhoeae had similarity index values in the range of inter-strain index values.
采用聚丙烯酰胺平板凝胶电泳法对19种奈瑟菌属细菌（包括6株浅黄奈瑟菌）、溶血孪生球菌、大肠埃希菌和卡他布兰汉菌的12种酶的电泳迁移率进行了表征。所有测试的菌株和菌种均呈现出定性不同的酶谱模式。通过对所有12种酶系统进行两两比较以获得相似性指数，从而对菌种和菌株间的关系进行量化；这些数据采用数值聚类方法以获得分组和系统树图。电泳分类结果与通过其他标准获得的结果相比具有优势。此外，定量聚类数据表明，绵羊奈瑟菌和豚鼠奈瑟菌与其他奈瑟菌属菌种差异足够大，足以将它们分为一个独特的组。这两个菌种也缺乏在所有其他奈瑟菌属菌种中发现的特征性NADPH-黄递酶酶谱模式。种内相似性指数通常大于种间指数值。然而，某些菌种，如脑膜炎奈瑟菌和淋病奈瑟菌，其相似性指数值处于菌株间指数值范围内。
