Jackson P, Thornley M J, Thompson R J
J Gen Microbiol. 1984 Dec;130(12):3189-201. doi: 10.1099/00221287-130-12-3189.
High-resolution two-dimensional polyacrylamide gel electrophoresis was used to analyse the soluble proteins from seven strains of Neisseria gonorrhoeae, six strains of Neisseria meningitidis and one or two strains of twelve other species. Approximately 200 individual polypeptides could be visualized as Coomassie Blue stained spots on an electrophoretogram of N. gonorrhoeae and similar numbers were found for the other bacteria. Each species of bacterium had a distinctly different pattern of spots which could be recognized. Quantitative comparisons of 48 selected spots derived from one strain of N. gonorrhoeae with those of five other strains of gonococcus, three strains of N. meningitidis and one of Branhamella catarrhalis, showed relationships in agreement with their current taxonomic classification but with a higher level of discrimination than that of previously used methods. It was also possible to distinguish the individual gonococcal strains. It is suggested that the method could be useful for bacterial classification and identification.
采用高分辨率二维聚丙烯酰胺凝胶电泳分析了7株淋病奈瑟菌、6株脑膜炎奈瑟菌以及其他12个菌种中的1株或2株的可溶性蛋白质。在淋病奈瑟菌的电泳图谱上，约200条单个多肽可显现为考马斯亮蓝染色斑点，其他细菌也发现了类似数量的斑点。每种细菌都有明显不同的斑点模式，可加以识别。对源自1株淋病奈瑟菌的48个选定斑点与其他5株淋球菌、3株脑膜炎奈瑟菌和1株卡他布兰汉菌的斑点进行定量比较，结果显示其关系与当前的分类学分类一致，但区分度比以前使用的方法更高。还能够区分各个淋球菌菌株。建议该方法可用于细菌分类和鉴定。
