Medentsev A G, Trutko S M, Akimenko V K
Mikrobiologiia. 1985 Nov-Dec;54(6):876-82.
The work is concerned with the effect produced by limiting the growth of various bacteria with carbon, sulfur and iron on cytochrome synthesis, development of cyanide-resistant respiration and oversynthesis of metabolites. The cessation of bacterial growth due to the exhaustion of a carbon source was shown to be accompanied with the development of cyanide-resistant respiration though the oversynthesis of metabolites did not occur. If the growth was limited by a sulfur or iron source, the concentration of cytochromes a, b and c fell down as compared with that when the growth was limited by a carbon source, and metabolites were produced and accumulated in the medium. In that case, the respiration of virtually all the bacteria was inhibited by cyanide to a great extent. As was demonstrated for Pseudomonas aeruginosa, the development of cyanide-resistant respiration was inhibited when metabolites accumulated and then the respiration became completely resistant to cyanide as soon as the oversynthesis ceased. Apparently, whatever limits the bacterial growth, the process of oversynthesis inhibits cyanide-resistant oxidase.
这项工作关注的是用碳、硫和铁限制各种细菌生长对细胞色素合成、抗氰呼吸的发展以及代谢产物过量合成所产生的影响。结果表明，由于碳源耗尽导致细菌生长停止时，会伴随着抗氰呼吸的发展，尽管代谢产物没有过量合成。如果生长受到硫源或铁源的限制，与受碳源限制时相比，细胞色素a、b和c的浓度会下降，并且代谢产物会在培养基中产生和积累。在这种情况下，几乎所有细菌的呼吸在很大程度上都受到氰化物的抑制。正如对铜绿假单胞菌所证明的那样，当代谢产物积累时，抗氰呼吸的发展会受到抑制，而一旦过量合成停止，呼吸就会完全抗氰。显然，无论限制细菌生长的是什么，过量合成过程都会抑制抗氰氧化酶。
