Akimenko V K, Medentsev A G
Biokhimiia. 1980 Aug;45(8):1433-41.
Changes in the activity of the cell respiration of the yeast Candida lipolytica and its ATP, ADP, NADH, NAD+ pools during the development of the cyanide-resistant respiration were studied. A change-over of the yeast culture to the stationary growth phase conditioned by glucose exhaustion or aerobic incubation of the resting cells in the exponential growth phase without the exogenous carbon source were shown to be accompanied by: 1) decrease of the rate of oxygen consumption; 2) appearance of the cyanide-resistant respiration; 3) appearance of the benzhydroxamic acid-sensitive respiration; 4) appearance of stimulating dinitrophenol action on the rate of oxygen consumption; 5) increase in the ATP content and decrease of the ADP content in the cells. It was concluded that the appearance of the cyanide-resistant respiration is induced by the decrease of the activity of the respiratory chain due to the increase of the ATP concentration and the decrease of the ADP concentration in yeast cells. The functioning of the cyanide-resistant pathway of the electron transfer is one of the ways of NAD+ pool regulation in yeast cells.
研究了解脂耶氏酵母细胞呼吸活性及其ATP、ADP、NADH、NAD⁺库在抗氰呼吸发展过程中的变化。结果表明，酵母培养物转变为静止生长阶段（由葡萄糖耗尽引起）或处于指数生长期的静止细胞在无外源碳源条件下进行需氧培养时，会伴随以下情况：1）耗氧速率降低；2）出现抗氰呼吸；3）出现对苯甲羟肟酸敏感的呼吸；4）出现二硝基苯酚对耗氧速率的刺激作用；5）细胞内ATP含量增加，ADP含量降低。得出的结论是，酵母细胞中由于ATP浓度升高和ADP浓度降低导致呼吸链活性下降，从而诱导了抗氰呼吸的出现。电子传递的抗氰途径的功能是酵母细胞中NAD⁺库调节的方式之一。
