Gilbert P, Dickinson N A, Brown M R
J Gen Microbiol. 1979 Nov;115(1):89-94. doi: 10.1099/00221287-115-1-89.
Escherichia coli W3110 was grown in a chemostat under conditions of carbon limitation at various temperatures and specific growth rates (mu). Exponential survivor-time curves following cold osmotic shock were biphasic. These could be described by the sum of two exponential functions representing the survival of sensitive and resistant fractions of the population where the size of the sensitive fraction was directly proportional to mu. Decimal reduction times for the more resistant fraction were unaffected by mu yet decreased with increasing growth temperature. Sensitivity to cold shock was evaluated for an E. coli CR34 mutant, temperature-sensitive in initiation of DNA replication. When grown in the chemostat at the non-restrictive temperature (30 degrees C) sensitivity was directly proportional to mu. Following a rise in the incubation temperature to 42 degrees C, sensitivity decreased markedly and reached a minimum 45 to 60 min after the temperature increase. Sensitivity of the E. coli mutant grown at 30 degrees C and raised to 42 degrees C for 1 h was low and relatively unaffected by growth rate.
大肠杆菌W3110在恒化器中于不同温度和比生长速率（μ）下在碳限制条件下培养。冷渗透休克后的指数存活时间曲线是双相的。这些曲线可以用两个指数函数的和来描述，这两个指数函数分别代表群体中敏感部分和抗性部分的存活情况，其中敏感部分的大小与μ成正比。抗性更强部分的十进制减少时间不受μ影响，但随生长温度升高而降低。对DNA复制起始温度敏感的大肠杆菌CR34突变体进行了冷休克敏感性评估。当在恒化器中于非限制温度（30℃）下培养时，敏感性与μ成正比。将培养温度升至42℃后，敏感性显著降低，并在温度升高后45至60分钟达到最低值。在30℃下生长并升至42℃1小时的大肠杆菌突变体的敏感性较低，且相对不受生长速率的影响。
