Bai Hua, Naidu Trisha, Anderson James B, Montemayor Hector, Do Camie, Ni Lina
School of Neuroscience, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States.
Front Cell Neurosci. 2024 Sep 23;18:1347460. doi: 10.3389/fncel.2024.1347460. eCollection 2024.
exhibits multiple highly sophisticated temperature-sensing systems, enabling its effective response and navigation to temperature changes. Previous research has identified three dorsal organ cool cells (DOCCs) in fly larvae, consisting of two A-type and one B-type cell with distinct calcium dynamics. When subjected to hypertonic conditions, calcium imaging shows that A-type DOCCs maintain their responses to cool temperatures. In contrast, a subset of B-type DOCCs does not exhibit detectable GCaMP baseline signals, and the remaining detectable B-type DOCCs exhibit reduced temperature responses. The activation of both A-type and B-type DOCCs depends on the same members of the ionotropic receptor (IR) family: IR21a, IR93a, and IR25a. A-type DOCCs exhibit a higher somal level of IR93a than B-type DOCCs. Overexpression of restores B-type calcium responses to cool temperatures, but not the proportion of B-type cells with a detectable GCaMP baseline, in a hypertonic environment, suggesting a selective role of IR93a in maintaining the temperature responses under hypertonic conditions. Our findings identify a novel function of B-type DOCCs in integrating temperature and tonic stimuli.
表现出多种高度复杂的温度传感系统，使其能够对温度变化做出有效反应并进行导航。先前的研究已经在果蝇幼虫中鉴定出三个背器官冷细胞（DOCCs），由两个A型细胞和一个B型细胞组成，它们具有不同的钙动力学。在高渗条件下，钙成像显示A型DOCCs保持对低温的反应。相比之下，一部分B型DOCCs没有可检测到的GCaMP基线信号，其余可检测到的B型DOCCs表现出降低的温度反应。A型和B型DOCCs的激活都依赖于离子型受体（IR）家族的相同成员：IR21a、IR93a和IR25a。A型DOCCs的IR93a体细胞水平高于B型DOCCs。在高渗环境中，IR93a的过表达恢复了B型细胞对低温的钙反应，但没有恢复具有可检测到的GCaMP基线的B型细胞的比例，这表明IR93a在高渗条件下维持温度反应中具有选择性作用。我们的研究结果确定了B型DOCCs在整合温度和张力刺激方面的新功能。
