Coelho Líris Marini Dias, da Fonseca Vanessa Maciel Braulio, Labadessa Ivana Golçalves, Salvador Sergio Luiz, Del Arco Mastrange Marina, Gembre Ana Flávia, Martins Núbia Sabrina, Bonato Vânia Luiza Deperon, Vianna Élcio Oliveira, Carvalho Borges Marcos
Departament of Internal Medicine, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, 14048-900, Brazil.
Department of Clinical Analyses, School of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, 14040-903, Brazil.
Probiotics Antimicrob Proteins. 2024 Oct 9. doi: 10.1007/s12602-024-10366-5.
The increase in the prevalence of asthma, particularly in urban communities, has encouraged investigations into preventive strategies. The hygiene theory proposes that early exposure to infections and unhygienic conditions during childhood influences immune system development, potentially protecting against allergic diseases. The mechanisms involved are related to alterations in the intestinal microbiota, such as with probiotics. This study aimed to evaluate the preventive effect of Lacticaseibacillus rhamnosus, Lacticaseibacillus paracasei, and Bifidobacterium animalis ssp. lactis, administered isolated or in combination, at various concentrations, on asthma in an animal model. Mice received two concentrations (1 × 10 and 1 × 10 CFU/ml) of three probiotics, isolated and in combination, over 26 consecutive days, initiating 10 days before sensitizing and challenging with ovalbumin. In vivo bronchial hyperresponsiveness and airway and lung inflammation were assessed. The administration of L. paracasei, L. rhamnosus, and B. animalis spp. lactis in different concentrations, isolated or in combination, did not reduce hyperresponsiveness and airway and lung inflammation. As probiotic effects are strain and dose-dependents, specific studies are necessary to assess the effect of different probiotic strains, doses, and regimes.
哮喘患病率的上升，尤其是在城市社区，促使人们对预防策略进行研究。卫生假说提出，儿童时期早期接触感染和不卫生的环境会影响免疫系统发育，有可能预防过敏性疾病。其中涉及的机制与肠道微生物群的改变有关，比如与益生菌有关。本研究旨在评估鼠李糖乳杆菌、副干酪乳杆菌和动物双歧杆菌亚种乳酸亚种单独或联合使用不同浓度时，对动物模型哮喘的预防作用。小鼠连续26天接受三种益生菌单独及联合的两种浓度（1×10和1×10 CFU/ml），在对卵清蛋白致敏和激发前10天开始给药。评估体内支气管高反应性以及气道和肺部炎症。不同浓度单独或联合使用副干酪乳杆菌、鼠李糖乳杆菌和动物双歧杆菌亚种乳酸亚种，并未降低高反应性以及气道和肺部炎症。由于益生菌的作用具有菌株和剂量依赖性，因此需要进行具体研究来评估不同益生菌菌株、剂量和给药方案的效果。
