Department of Chemistry, State University of Midwest (UNICENTRO), Guarapuava, Paraná, Brazil.
Proteomics and Metabolomics Facility, Faculty of Medicine, Hammersmith Hospital Campus, Imperial College London, United Kingdom.
J Pharm Biomed Anal. 2025 Jan 1;252:116497. doi: 10.1016/j.jpba.2024.116497. Epub 2024 Oct 5.
Native stingless bees (Meliponini) from Brazil make (geo)propolis which is largely used in folk medicine, specially by indigenous and quilombos communities and beekeepers´ families but are progressively being recognized for their pharmacological activities. In this study, the ethanolic extracts of (geo)propolis (EEGs) from Melipona marginata, M. quadrifasciata, M. scutellaris, and Tetragonisca angustula were analysed by Flow injection analysis (FIA) and Ultra-high performance liquid chromatography (UHPLC) in a high resolution Orbitrap mass analyser (HRMS) to investigate and compare their chemical profile. Untargeted metabolomic approach based on UHPLC-HRMS experiments, and bioinformatic tools, allowed to annotate 59 compounds from diverse classes such as: flavonoids, phenolic compounds, sugars, terpenoids, and lipids. In addition, using multivariate tools and Flow injection- high resolution mass spectrometry (FIA-HRMS), it was possible to classify samples and identify marker ions related to the bee species or genus and to the geographical origin as a proof of concept.
巴西本土无刺蜜蜂（Meliponini）所产的（地）蜂胶在民间医学中被广泛应用，特别是在土著和夸拉多社区以及养蜂人家中，但它们的药理活性正逐渐得到认可。在这项研究中，利用流动注射分析（FIA）和超高效液相色谱（UHPLC）在高分辨率轨道阱质谱仪（HRMS）中对 M. marginata、M. quadrifasciata、M. scutellaris 和 Tetragonisca angustula 的（地）蜂胶的乙醇提取物（EEGs）进行了分析，以调查和比较它们的化学特征。基于 UHPLC-HRMS 实验和生物信息学工具的非靶向代谢组学方法，鉴定出了来自不同类别的 59 种化合物，如：类黄酮、酚类化合物、糖、萜类化合物和脂质。此外，使用多元工具和流动注射-高分辨率质谱（FIA-HRMS），可以对样本进行分类，并识别与蜜蜂种类或属以及地理来源相关的标记离子，以此作为概念验证。
