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超高效液相色谱-四极杆飞行时间质谱联用及核磁共振法快速测定马来西亚无刺蜜蜂蜂胶乙醇提取物中的主要成分。

Rapid Determination of Major Compounds in the Ethanol Extract of Geopropolis from Malaysian Stingless Bees, Heterotrigona itama, by UHPLC-Q-TOF/MS and NMR.

机构信息

Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.

Institute of Analytical Science, Shaanxi Provincial Key Lab of Electroanalytical Chemistry, Northwest University, Xi'an 710069, China.

出版信息

Molecules. 2017 Nov 10;22(11):1935. doi: 10.3390/molecules22111935.

DOI:10.3390/molecules22111935
PMID:29125569
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6150372/
Abstract

A reliable, rapid analytical method was established for the characterization of constituents of the ethanol extract of geopropolis (EEGP) produced by Malaysian stingless bees--by combining ultra-high-performance liquid chromatography with quadruple time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). Based on known standards, the online METLIN database, and published literature, 28 compounds were confirmed. Phenolic acids, flavones, triterpenes and phytosterol were identified or tentatively identified using characteristic diagnostic fragment ions. The results indicated that terpenoids were the main components of EEGP, accompanied by low levels of phenolic acids, flavonoids, and phytosterol. Two major components were further purified by preparative high-performance liquid chromatography (PHPLC) and identified by nuclear magnetic resonance (NMR) as 24()-cycloart-24-ene-26-ol-3-one and 20-hydroxy-24-dammaren-3-one. These two triterpenes, confirmed in this geopropolis for the first time, are potential chemical markers for the identification of geopropolis from Malaysian stingless bees, .

摘要

建立了一种可靠、快速的分析方法，用于结合超高效液相色谱与四极杆飞行时间质谱（UHPLC-Q-TOF/MS）分析马来西亚无刺蜜蜂产的蜂胶醇提物（EEGP）的成分特征。基于已知标准、在线 METLIN 数据库和已发表的文献，鉴定出了 28 种化合物。使用特征诊断碎片离子，鉴定或初步鉴定了酚酸、黄酮类、三萜和植物甾醇。结果表明，萜类是 EEGP 的主要成分，其次是低水平的酚酸、黄酮类和植物甾醇。通过制备高效液相色谱（PHPLC）进一步纯化了两种主要成分，并通过核磁共振（NMR）鉴定为 24()-环阿尔特-24-烯-26-醇-3-酮和 20-羟基-24-达玛烷-3-酮。这两种三萜类化合物首次在这种蜂胶中得到确认，是鉴定马来西亚无刺蜜蜂产蜂胶的潜在化学标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fac/6150372/1b9614b1e357/molecules-22-01935-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fac/6150372/8a356e7ed3cf/molecules-22-01935-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fac/6150372/28d65c577e7c/molecules-22-01935-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fac/6150372/b326592a4528/molecules-22-01935-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fac/6150372/a9f6d020b424/molecules-22-01935-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fac/6150372/1b9614b1e357/molecules-22-01935-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fac/6150372/8a356e7ed3cf/molecules-22-01935-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fac/6150372/28d65c577e7c/molecules-22-01935-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fac/6150372/b326592a4528/molecules-22-01935-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fac/6150372/a9f6d020b424/molecules-22-01935-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fac/6150372/1b9614b1e357/molecules-22-01935-g005.jpg

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