Park Yu-Jin, Kim Yoon-Young, Chang Ji-Youn, Kho Hong-Seop
Department of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, South Korea (ROK).
Department of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, South Korea (ROK); Institute of Aging, Seoul National University, Seoul, South Korea (ROK).
Int Dent J. 2025 Apr;75(2):1261-1268. doi: 10.1016/j.identj.2024.09.006. Epub 2024 Oct 9.
This study aimed to investigate the effects of zinc compounds on the candidacidal activities of lysozyme and the peroxidase (PO) and glucose oxidase-mediated peroxidase (GO-PO) systems against Candida albicans.
Four zinc compounds were used: zinc chloride, gluconate, lactate, and sulphate. Three antimicrobial systems were used: hen egg-white lysozyme (HEWL), the PO system [bovine lactoperoxidase (bLPO), potassium thiocyanate, and hydrogen peroxide], and the GO-PO system (bLPO, potassium thiocyanate, glucose oxidase, and glucose). Three Candida albicans strains were used: ATCC 10231, 11006, and 18804. The candidacidal activity of each zinc compound-antimicrobial system mixture was compared with that of the zinc compound or antimicrobial system alone.
The addition of zinc chloride and gluconate significantly (P < .05) increased the candidacidal activity of HEWL in the ATCC 10231 and 11006 strains. Regarding the PO system, the addition of zinc sulphate in the ATCC 10231 strain and of zinc chloride or gluconate in the ATCC 18804 strain significantly (P < .05) increased the candidacidal activity compared with the PO system alone. No significant changes were observed in the candidacidal activities of the mixture of each zinc compound and the GO-PO system compared with the GO-PO system alone for all three C. albicans strains.
Although it depended on the type of zinc compound or strain, the addition of zinc compounds increased the candidacidal activities of antimicrobial enzymes against C. albicans compared with HEWL or the PO system alone. The introduction of zinc compounds into oral healthcare products containing antimicrobials could provide additional antifungal activity.
本研究旨在调查锌化合物对溶菌酶以及过氧化物酶（PO）和葡萄糖氧化酶介导的过氧化物酶（GO-PO）系统抗白色念珠菌活性的影响。
使用了四种锌化合物：氯化锌、葡萄糖酸锌、乳酸锌和硫酸锌。使用了三种抗菌系统：鸡蛋清溶菌酶（HEWL）、PO系统[牛乳铁蛋白（bLPO）、硫氰酸钾和过氧化氢]以及GO-PO系统（bLPO、硫氰酸钾、葡萄糖氧化酶和葡萄糖）。使用了三株白色念珠菌：ATCC 10231、11006和18804。将每种锌化合物-抗菌系统混合物的杀念珠菌活性与单独的锌化合物或抗菌系统的活性进行比较。
添加氯化锌和葡萄糖酸锌显著（P <.05）提高了HEWL对ATCC 10231和11006菌株的杀念珠菌活性。对于PO系统，与单独的PO系统相比，在ATCC 10231菌株中添加硫酸锌以及在ATCC 18804菌株中添加氯化锌或葡萄糖酸锌显著（P <.05）提高了杀念珠菌活性。对于所有三株白色念珠菌，与单独使用GO-PO系统相比，每种锌化合物与GO-PO系统混合物的杀念珠菌活性未观察到显著变化。
尽管这取决于锌化合物的类型或菌株，但与单独的HEWL或PO系统相比，添加锌化合物可提高抗菌酶对白色念珠菌的杀念珠菌活性。将锌化合物引入含抗菌剂的口腔护理产品中可提供额外的抗真菌活性。
