Hwang Yoon-Jai, Kim Yoon-Young, Chang Ji-Youn, Kho Hong-Seop
Department of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
Institute on Aging, Seoul National University, Seoul, Republic of Korea.
J Dent Sci. 2025 Apr;20(2):885-894. doi: 10.1016/j.jds.2024.09.002. Epub 2024 Sep 16.
BACKGROUND/PURPOSE: Given the antifungal activity of zinc compounds against , this study aimed to investigate the effects of incubation time, pH, and ionic strength on their candidacidal activity against .
Two zinc compounds (zinc chloride and sulfate) and three . strains (ATCC 10231, 11006, and 18804) were used. The parameters considered include incubation times of immediate, 10, 60, and 120 min, pH of 5.5, 5.0, 4.5, and 4.0, and ionic strength of mimicking human saliva, 100, 150, and 200 mM. The candidacidal activity was determined by comparing colony numbers on the experimental and control plates.
The candidacidal activities of the zinc compounds significantly increased with increasing incubation time, decreasing pH, and increasing ionic strength. Significant levels of candidacidal activity were detected even within 10 min of incubation. The zinc compounds provided additional candidacidal activity under each pH and ionic strength condition. However, the effects of adding zinc compounds were less than those of lowering pH or increasing ionic strength. The candidacidal activities at each incubation time, pH, and ionic strength varied depending on the zinc compound and strain; lower activities in zinc chloride and the 18804 strain.
The candidacidal activity of the zinc compounds occurred immediately. They provided additional candidacidal activities even at low pH or high ionic strength environments. Oral healthcare products containing zinc compounds can provide antifungal activities in a changing oral environment.
背景/目的：鉴于锌化合物对[具体对象未明确]具有抗真菌活性，本研究旨在探究孵育时间、pH值和离子强度对其抗[具体对象未明确]活性的影响。
使用了两种锌化合物（氯化锌和硫酸锌）以及三株[具体菌株未明确]菌株（ATCC 10231、11006和18804）。所考虑的参数包括即时、10、60和120分钟的孵育时间，5.5、5.0、4.5和4.0的pH值，以及模拟人类唾液、100、150和200 mM的离子强度。通过比较实验平板和对照平板上的菌落数来确定抗[具体对象未明确]活性。
锌化合物的抗[具体对象未明确]活性随孵育时间增加、pH值降低和离子强度增加而显著提高。即使在孵育10分钟内也检测到显著水平的抗[具体对象未明确]活性。在每种pH值和离子强度条件下，锌化合物都提供了额外的抗[具体对象未明确]活性。然而，添加锌化合物的效果小于降低pH值或增加离子强度的效果。在每个孵育时间、pH值和离子强度下，抗[具体对象未明确]活性因锌化合物和[具体菌株未明确]菌株而异；氯化锌和18804菌株中的活性较低。
锌化合物的抗[具体对象未明确]活性立即出现。即使在低pH值或高离子强度环境中，它们也提供了额外的抗[具体对象未明确]活性。含有锌化合物的口腔护理产品可以在不断变化的口腔环境中提供抗真菌活性。
