State Key Laboratory of Subtropical Forest Cultivation, Zhejiang A & F University, Dong Hu Campus, 666 Wusu Street, Lin'an, 311300, China.
Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang A & F University, Dong Hu Campus, 666 Wusu Street, Lin'an, 311300, China.
BMC Plant Biol. 2024 Oct 9;24(1):938. doi: 10.1186/s12870-024-05664-7.
Botryosphaeria dothidea is the main fungal pathogen responsible for causing Chinese hickory (Carya cathayensis) dry rot disease, posing a serious threat to the Chinese hickory industry. Understanding the molecular basis of B. dothidea infection and the host's resistance mechanisms is crucial for controlling and managing the ecological impact of Chinese hickory dry rot disease. This study utilized ultrastructural observations to reveal the process of B. dothidea infection and colonization in Chinese hickory, and investigated the impact of B. dothidea infection on Chinese hickory biochemical indicators and plant hormone levels. Through high-throughput transcriptome sequencing, the gene expression profiles associated with different stages of B. dothidea infection in Chinese hickory and their corresponding defense responses were described. Additionally, a series of key genes closely related to non-structural carbohydrate metabolism, hormone metabolism, and plant-pathogen interactions during B. dothidea infection in Chinese hickory were identified, including genes encoding DUF, Myb_DNA-binding, and ABC transporter proteins. These findings provide important insights into elucidating the pathogenic mechanisms of B. dothidea and the resistance genes in Chinese hickory.
球腔菌是引起中国山核桃干腐病的主要真菌病原体，对中国山核桃产业构成严重威胁。了解 B. dothidea 感染的分子基础和宿主的抗性机制对于控制和管理中国山核桃干腐病的生态影响至关重要。本研究利用超微结构观察揭示了 B. dothidea 感染和定殖中国山核桃的过程，并研究了 B. dothidea 感染对中国山核桃生化指标和植物激素水平的影响。通过高通量转录组测序，描述了与 B. dothidea 感染中国山核桃不同阶段相关的基因表达谱及其相应的防御反应。此外，还鉴定了一系列与 B. dothidea 感染中国山核桃过程中非结构性碳水化合物代谢、激素代谢和植物-病原体相互作用密切相关的关键基因，包括编码 DUF、Myb_DNA 结合和 ABC 转运蛋白的基因。这些发现为阐明 B. dothidea 的致病机制和中国山核桃的抗性基因提供了重要的见解。
