Maslahat Mamay, Mardinata Dion, Surur Siti Maspupatu, Lioe Hanifah Nuryani, Syafitri Utami Dyah, Rafi Mohamad, Rohaeti Eti
Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, 16680, Indonesia.
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Nusa Bangsa University, Bogor, 16166, Indonesia.
Chem Biodivers. 2025 Feb;22(2):e202401537. doi: 10.1002/cbdv.202401537. Epub 2024 Nov 26.
The composition and concentration of compounds in medicinal plants vary based on several factors, including the specific part of the plant being used. These variations in composition and concentration lead to differences in biological activity levels. In this study, we aimed to assess the phytochemical profile of Sonchus arvensis and to investigate the biological activity of different plant parts (roots, stems, and leaves) using a metabolomics approach. We analyzed the plant extracts for total phenolic and flavonoid levels, antioxidant activity, and xanthine oxidase inhibition. We also conducted metabolite profiling using Fourier-transform infrared spectroscopy and liquid chromatography-high resolution mass spectrometry. A total of 17 metabolites were identified (13 in leaves, 10 in stems, and 9 in roots). Principal component analysis effectively differentiated S. arvensis extracts based on differences in plant parts. These findings indicate that the quantity and diversity of metabolites present in the roots, stems, and leaves influence the biological activity of S. arvensis.
药用植物中化合物的组成和浓度会因多种因素而有所不同，包括所使用的植物特定部位。这些组成和浓度的变化导致生物活性水平的差异。在本研究中，我们旨在评估苣荬菜的植物化学特征，并使用代谢组学方法研究不同植物部位（根、茎和叶）的生物活性。我们分析了植物提取物中的总酚和黄酮水平、抗氧化活性以及黄嘌呤氧化酶抑制作用。我们还使用傅里叶变换红外光谱和液相色谱 - 高分辨率质谱进行了代谢物谱分析。总共鉴定出17种代谢物（叶中13种，茎中10种，根中9种）。主成分分析基于植物部位的差异有效地区分了苣荬菜提取物。这些发现表明，根、茎和叶中存在的代谢物的数量和多样性会影响苣荬菜的生物活性。
